中国临床药理学与治疗学2016，Vol.21Issue(5)：486-489,502,5.
地高辛抗血清对缺血再灌注心肌细胞凋亡的影响及机制
Effects and mechanism of anti-digoxin antiserum on apoptosis in ischemia/reperfusion cardiomyocytes
摘要
Abstract
AIM:To investigate the effects and mechanism of anti-digoxin antiserum on the apoptosis induced by ischemia/reperfusion (I/R) in neonatal cardiomyocytes.METHODS:Primary neonatal rat cardiomyocytes were incubated in ischemia mimetic solution for 2 h,and then reincubated in normal culture medium for 24 h to establish a model of ischemia/reperfusion.The cardiomyocytes were randomly divided into:control group,I/R group,Oua + I/R group,and DigAb + I/R group.Cardiomyocyte apoptosis was detected by Annexin V-FITC/PI.The expressions of endoplasmic reticulum stress associated protein GRP78 and CHOP were analyzed by Western blot.RESULTS:Compared with the control group,apoptosis cardiomyocytes were significantly increased in I/R and Oua + I/R groups (P ＜ 0.001).Treatment of anti-digoxin antiserum decreased I/R-induced apoptosis cardiomyocytes (P ＜ 0.001).The expressions of GRP78 and CHOP were significantly increased in I/R and Oua + I/R groups (P ＜ 0.01).The expressions of GRP78 and CHOP were inhibited by treatment of anti-digoxin antiserum (P ＜ 0.05).CONCLUSION:Anti-digoxin antiserum decrease cardiomyocyte apoptosis induced by I/R,and its mechanism may be partly by inhibiting endoplasmic reticulum stress.关键词
心肌细胞/凋亡/内质网应激/缺血再灌注Key words
cardiomyocyte/apoptosis/endoplasmic reticulum stress/ischemia/reperfusion分类
医药卫生引用本文复制引用
汪和贵,张明超,胡雨龙,柯永胜..地高辛抗血清对缺血再灌注心肌细胞凋亡的影响及机制[J].中国临床药理学与治疗学,2016,21(5):486-489,502,5.基金项目
安徽省自然基金面上项目(1308085MH116) （1308085MH116）
安徽省教育厅自然科学重大项目(KJ2015ZD42) （KJ2015ZD42）
