中国临床药理学与治疗学2016，Vol.21Issue(5)：495-502,8.
黄连素调节2型糖尿病地鼠内脏脂肪组织FGF21/SIRT1信号通路基因mRNA表达的效应
The effects on regulations of berberine on gene mRNA expression of FGF21/SIRT1 signal pathway in visceral adipose tissue from type 2 diabetic hamsters
摘要
Abstract
AIM:To investigate the effects of berberine on the gene mRNA expression of FGF21/SIRT1/PRDM16 signal pathway and PRDM16 signal pathway in visceral white adipose tissues (VWAT) from obesity-related type 2 diabetic Chinese hamsters and the related mechanisms.METHODS:The obese insulin-resistant hamster models were induced by high-fat diet and type 2 diabetic hamster models were induced by obese insulin-resistant hamster models injected with low-dose streptozotocin.After that,the hamsters were randomly divided into control,obese insulin-resistant,obese type 2 diabetic and berberine-treated type 2 diabetic groups.After nine-week berberine treatment,the gene mRNA expression changes of VWAT FGF21/SIRT1/PRDM16 signal pathway and PRDM16 signal pathway from different groups were measured by using real-time RT-PCR.RESULTS:The gene mRNA expression of FGF21,FGFR1,βKL,SIRT1,GLUT-1,PRDM16,CtBP-1,CtBP-2,C/EBPβ,PPARγ,PGC-1α,PGC-1β and brown adipose tissue-specific genes such as UCP-1,Cidea,Elovl3 and PPARα was decreased and that of white adipose tissue-specific genes including Resistin,MEST and Serpina3k was increased in dysfunctional VWAT from hamster models.Berberine treatment induced VWAT FGF21/SIRT1/PRDM16 signal pathway and PRDM16 signal pathway,induced the gene mRNA expression of brown adipose tissue-specific genes and inhibited that of white adipose tissue-specific genes,induced browning of white adipose tissues and improved fat-induced insulin resistance.CONCLUSION:FGF21/SIRT1/PRDM16 signal pathway and PRDM16 signal pathway involved in the inductive molecular mechanisms of berberine on browning of visceral white adipose tissues in type 2 diabetic hamsters.关键词
黄连素/2型糖尿病/FGF21/SIRT1/内脏白色脂肪组织棕色化Key words
berberine/type 2 diabetes/FGF21/SIRT1/browning of visceral white adipose tissues分类
医药卫生引用本文复制引用
刘栩晗,李国生,李欣宇,高政南,黄澜,刘亚莉..黄连素调节2型糖尿病地鼠内脏脂肪组织FGF21/SIRT1信号通路基因mRNA表达的效应[J].中国临床药理学与治疗学,2016,21(5):495-502,8.基金项目
中华医学会临床医学科研专项资金项目(12020200300) （12020200300）
大连市科学技术项目(2013) （2013）
