中国临床药理学与治疗学2016，Vol.21Issue(5)：503-508,6.
天麻素联合电针调节局灶性脑缺血大鼠额叶皮质GFAP和NSE的表达
Effects of electroacupuncture combined with gastrodin on the expression of GFAP and NSE in the dentate gyrus of focal cerebral ischemia rats
摘要
Abstract
AIM:To observe the effect of electroacupuncture (EA) combined with gastrodin on the expression of glial fibrilally acidic protein (GFAP) and neuron specific enolase (NSE) in the dentate gyms (DG) of focal cerebral ischemia(FCI) rats,so as to explore its mechanism underlying improvement of differentiation at Hippocampal eNSCs of FCI.METHODS:Fifty Sprague-Dawley rats were randomly divided into the normal,model,EA,gastrodin and EA + gastrodin groups.FCI rats were induced by middle cerebral artery occlusion (MCAO) with thread embolus.The gastrodin group was injected with a daily 10 mg/kg dose of gastrodin intraperitoneally for 14 days.The EA group was given daily 30 min electro-acupuncture on left side “ Baihui ”(DU20) and “Zusanli” (ST36) for 14 days.The EA + gastrodin group was given a combined treatment of gastrodin and electro-acupuncture for 14 days.The expressions of GFAP and NSE in DG were detected by immunohistochemical and image analysis system.RESULTS:Compared with the normal group,the GFAP expression level of the model group was significantly increased (P ＜ 0.05),while the NSE expression level was significantly decreased (P ＜ 0.05).Compared with the model group,the GFAP expression levels were down-regulated in the EA,gastrodin and EA + gastrodin group (P ＜0.05),while the NSE expression levels were decreased.Compared with the gastrodin or the EA team,the GFAP expression level of the EA + gastrodin team was decreased while the NSE expression level was increased (P ＜ 0.05).CONCLUSION:Both EA and gastrodin can significantly promoted eNSCs differentiation into neurons instead of glial cells.The effect is more obvious in the EA + gastrodin group,which may be one of the possible mechanisms underlying its good resistance to cerebral ischemia injury.关键词
局灶性脑缺血/天麻素/电针/胶质纤维酸性蛋白/神经元特异性烯醇化酶Key words
focalcerebral ischemia/electroacupuncture/gastrodin/glial fibrilally acidic protein/neuron-specific enolase分类
医药卫生引用本文复制引用
李怀斌,吴锋,缪化春,熊克仁,赵健,黄锐..天麻素联合电针调节局灶性脑缺血大鼠额叶皮质GFAP和NSE的表达[J].中国临床药理学与治疗学,2016,21(5):503-508,6.基金项目
安徽省高等学校自然科学研究重点项目(KJ2015A227) （KJ2015A227）
皖南医学院重点科研项目培育基金(WK2014207) （WK2014207）
