中国临床药理学杂志2016，Vol.32Issue(12)：1059-1062,4.DOI:10.13699/j.cnki.1001-6821.2016.12.001
脓毒症患者血清诱导血管内皮细胞通透性相关因素研究
Study on relationship between vascular endothelial cells hyperpermeability induced by serum of patients with sepsis and relative factors
摘要
Abstract
Objective To study the effect of serum ( 20%) of patients with sepsis on human vascular endothelial cell (ECs) monolayer permea-bility.Methods Selected 45 cases of sepsis were enrolled as the experi-ment group , according to processing methods were divided into experi-ment groupⅠ(n=32, no treatment) and experiment groupⅡ(n=23, Y27632 ) and 40 cases of healthy volunteers were divided into the normal groupⅠ(n=20, no treatment) and normal group Ⅱ(n=20,Y27632), then to 20%of patients with sepsis serum stimulation endothelial cells 6 h.Determination of endothelial cell monolayer permeability observed en-dothelial cell morphology distribution and detection of RhoA ( RAS homo-log A ) protein expression.Results The permeability of experimentⅠgroup was significantly higher than that of the normal group.But the higher permeability in experiment Ⅱgroup was inhibited after pretreated with Y27632 and the difference was statistically significant ( P<0.05 ).The inhibitor Y27632 ( normal group Ⅱ) was not effect on permeability . The serum of the patients with sepsis ( two experiment groups ) could increase the number of intercellular spaces , change the permeability of the endothelial cells , and effectively induced the expression of RhoA protein.Conclusion Serum of patients with sepsis significantly induced endothelial cell permeability increase .One of its mechanisms involved the regulation role of Ras homolog /Rho-associated protein kinase ( Rho∕ROCK) signaling pathway , which could damage integrity of endothelial barrier function.关键词
脓毒症/人血管内皮细胞/细胞通透性Key words
sepsis/human vascular endothelia/cell permeability分类
医药卫生引用本文复制引用
郑运江,胡晓峰,陈庆青,王彩平,化学胜,章韵,汤耀卿..脓毒症患者血清诱导血管内皮细胞通透性相关因素研究[J].中国临床药理学杂志,2016,32(12):1059-1062,4.基金项目
上海市卫生计生委基金资助项目（20124318）；上海交通大学科技发展处基金资助项目（12 XJ22016）；崇明县科技发展基金资助项目 （）
