中国临床药理学杂志2016，Vol.32Issue(12)：1101-1103,3.DOI:10.13699/j.cnki.1001-6821.2016.12.014
肝细胞生长因子对大鼠心肌细胞动作电位的作用
Effect of hepatocyte growth factor on the action potential duration of ventricular myocytes in rat
摘要
Abstract
Objective To study the effect of hepatocyte growth factor ( HGF) on the action potential duration ( APD) of ventricular myocytes in rat.Methods Myocytes were isolated by collagenase and were randomly divided into control group ( HGF,0 ) and three concentration test groups (5, 10, 50 μg· mL-1 HGF).With isoprenaline ( ISO) as stimulating factor , patch clamp technique was used to record the APD 50 and APD90 in cells incubated by HGF.Results APD in control group were significantly prolonged by ISO.Compared with the control group , the prolongation of APD50 in small, middle and large test groups decreased 1.2%, 42.3% and 49.2%, while the one of APD90 decreased 19%, 41.1% and 48.8%, respectively as the increasing dose of HGF.The effect of HGF against ISO tends to be concentration dependent.Without ISO, APD90 in three test groups were shortened 6.8%, 13.3% and 28.2%respectively by HGF compared with control group , as the similar trend.Conclusion HGF could shorten APD and promote repolarization , which might have the potential to resist the electrical remodeling when cardiac hypertrophy that was helpful to the treatment of cardiac hypertrophy.关键词
肝细胞生长因子/心肌细胞/动作电位时程/异丙肾上腺素Key words
hepatocyte growth factor/cardiomyocyte/action potential duration/isoprenaline分类
医药卫生引用本文复制引用
曾凯,耿帅,董丽萍,赵育林,蒋园,龚冬梅..肝细胞生长因子对大鼠心肌细胞动作电位的作用[J].中国临床药理学杂志,2016,32(12):1101-1103,3.基金项目
国家自然科学基金资助项目（81202523）；黑龙江省高校战略后备人才出国研修基金资助项目 （）
