中国临床药理学杂志2016，Vol.32Issue(12)：1112-1114,3.DOI:10.13699/j.cnki.1001-6821.2016.12.017
牛蒡子苷元对人Ⅱ型子宫内膜癌细胞增殖抑制的研究
Effects of arctigenin on inhibiting proliferation of type Ⅱ endometrial carcinoma cells
徐军娟 1裘雅芬 1冯燕1
作者信息
- 1. 杭州师范大学 附属医院 妇产科，杭州310015
- 折叠
摘要
Abstract
Objective To explore the effects of arctigenin on inhibiting proliferation of type Ⅱ endometrial carcinoma cells , and its relationship with vascular endothelial growth factor ( VEGF).Methods The typeⅡendometrial carcinoma cells line were divided into control group , test A group and test B group.The control group were cultured for 7 days without any drugs.The test A group and test B group were treated with Arctigenin for 7 days at the concentration of 10 μmol · L-1 and 20 μmol· L-1 respectively.Cell proliferation and inhibition level were measured by cell counting kit ( CCK -8 ) and cell growth curve.The expression of mRNA and protein for VEGF were measured by reverse transcription polymerase chain reaction and Western blot.Results Comparing with the control group , the test A group and test B group have inhibitory effects on proliferation of type Ⅱendometrial carci-noma cell line ( P<0.05 ).The effect of inhibition in test B group was more obvious than in test A group ( P<0.01 ).The expression of mRNA and protein for VEGF in test A group and test B group decreased ( P<0.05 ).The expression level in test B group decreased more significantly than in test A group ( P<0.01 ).Conclusion The effect of Arctigenin on inhibiting proliferation of typeⅡendometrial carcinoma cells is implemented by down-regulating the genetic expression of VEGF.关键词
牛蒡子苷元/Ⅱ型子宫内膜癌/细胞增殖/血管内皮生长因子Key words
arctigenin/type Ⅱendometrial cancer/cell proliferation/vascular endothelial growth factor分类
医药卫生引用本文复制引用
徐军娟,裘雅芬,冯燕..牛蒡子苷元对人Ⅱ型子宫内膜癌细胞增殖抑制的研究[J].中国临床药理学杂志,2016,32(12):1112-1114,3.
