中国临床药理学杂志2016，Vol.32Issue(12)：1115-1117,3.DOI:10.13699/j.cnki.1001-6821.2016.12.018
丙泊酚对谷氨酸诱导的 PC12细胞凋亡的影响
Effect of propofol on the apoptosis of PC12 cell induced by glutamic acid
摘要
Abstract
Objective To explore the effect of propofol on the apoptosis of PC12 cell induced by glutamic acid.Methods PC12 cells were in-duced 10 mmol · L-1 glutamate for 48 h, and were divided into model group , propofol low , medium and high dose groups.The normal cells were used as control group.Control group and model group were received culture medium without any drugs for 48 h.Propofol low , medium and high dose groups were given 12.5 , 25.0 , 50.0 μmol · L-1 propofol for 48 h.The viability of PC12 cell was measured by MTT assay.PC12 cell apoptosis was measured by flow cytometry.The activity of Caspase -3 was determined by spectrophotometric method.The expression of FBJ osteosarcoma oncogene ( c -fos ) and early growth response protein 1 ( Egr -1 ) were detected by Realtime -PCR and western blot.Results Compared with the normal group , the cell apoptosis rate , the activity of Caspase -3 , the expression of c -fos mRNA and protein increased , and the cell viability , the expression of Egr -1 mRNA and protein decreased in the model group ( P<0.01 ).Compared with the model group, the acitivity of Caspase -3, the cell apoptosis rate , the activity of Caspase -3, the expression of c-fos mRNA and protein decreased , and the cell viability , the expression of Egr-1 mRNA and protein increased in propofol low -dose , medium -dose and high -dose group ( P <0.05 ).Conclusion Propofol suppressed the apoptosis of PC 12 cell induced by glutamic acid , which was related with the expression of c-fos and Egr-1.关键词
丙泊酚/PC12细胞/凋亡/即刻早基因/早期生长反应因子基因-1Key words
propofol/PC12 cell/cell apoptosis/FBJ osteosarcoma oncogene/early growth response protein -1分类
医药卫生引用本文复制引用
李峥,司金春,高项羽,刘喆,梁楠,南征..丙泊酚对谷氨酸诱导的 PC12细胞凋亡的影响[J].中国临床药理学杂志,2016,32(12):1115-1117,3.基金项目
河南省医学教育教学改革项目基金资助项目 （）
