中国临床药理学杂志2016，Vol.32Issue(12)：1121-1124,4.DOI:10.13699/j.cnki.1001-6821.2016.12.021
小檗碱配伍水苏糖对糖尿病小鼠糖脂代谢及肠道菌群的影响
Effects of berberine compatible with stachyose on glucolipid metabolism and gut microbiota in diabetic mice
摘要
Abstract
Objective To evaluate the effects of berberine compatible with stachyose on glucolipid metabolism and gut microbiota in type 2 dia-betic KKAy mice.Methods Female diabetic KKAy mice selected by insulin tolerance test (ITT) were divided into control group ( water 0.1 mL· 10 g -1 ), berberine group ( berberine 100 mg · kg -1 · d-1 ), stachyose group ( stachyose 200 mg · kg -1 · d-1 ) and combined group ( berberine 100 mg· kg-1 · d -1 +stachyose 200 mg · kg -1 · d -1 ) , 9 mice per group.Ten female C57BL/6J mice were served as normal con-trol group ( water 0.1 mL· 10 g -1 ).All mices were orally administrated once daily for 7 weeks.Fasting plasma glucose and lipids levels were detected.Oral glucose tolerance test ( OGTT) and ITT were determined at the end of treatment.In addition, the quantities of Lactobacillus and Bifidobacterium in mices intestine were examined by 16 SrRNA-targeted real -time fluorescence quantitative polymerase chain reaction.Results Compared with control group , combined group was markedly improved glucose tolerance and insulin sensitivity ( P<0.01 ) , decreased fasting plasma glucose and total cholesterol levels ( P <0.05 ) and enriched the abundance of Lactobacillus ( P<0.01 ) and Bifidobacterium ( P<0.01 ) , which were significantly better than berberine group and stachyose group.Conclusion Berberine combined with stachyose provided a better control for glucolipid metabolism than any single component in diabetic KKAy mice , which was related to up -regulation of Lactobacillus and Bifidobacterium in mice intestine.关键词
小檗碱/水苏糖/糖脂代谢/肠道菌群Key words
berberine/stachyose/glucolipid metabolism/gut microbiota分类
医药卫生引用本文复制引用
韩雨薇,李彩娜,环奕,孙素娟,穆永钊,申竹芳..小檗碱配伍水苏糖对糖尿病小鼠糖脂代谢及肠道菌群的影响[J].中国临床药理学杂志,2016,32(12):1121-1124,4.基金项目
重大新药创制国家科技重大专项基金资助项目 （）
