中国临床药理学杂志2016，Vol.32Issue(12)：1125-1128,4.DOI:10.13699/j.cnki.1001-6821.2016.12.022
狼疮清颗粒对系统性红斑狼疮的动物实验研究
Study on the effects of Langchuangqing granule on systemic lupus erythematosush in animals
摘要
Abstract
Objective To observe the effects of Langchuangqing granule on the Forssman cutaneous vasculitis reaction in guinea pigs , the antibo-dies, complements and immunoglobulin levels in MRL/lpr mice and the antibodies levels in the spleen lymphocytes.Methods In the forssman cutaneous vasculitis reaction , the immune serums were intradermally in-jected to the back skin of guinea pigs followed by tail vein injection of 0.5%evans blue.The skins dyed evans blue showed the model estab-lished.The guinea pigs were divided into five groups:model group , con-trol group and high-dose, middle-dose, low-dose ( Langchuangqing granule,18.27, 9.13, 4.56 g· kg -1) of test groups.The optical densi-ty( OD) of the skin was tested after continuously intragastric administra-tion for 8 days.In the lupus experiment in MRL/lpr mice, the models were established naturally with the mice growing up.The mice were divided into six groups: blank group , model group , control group and high-dose, middle-dose, low-dose(Langchuangqing granule,45.67, 22.83, 11.42 g· kg -1) of test groups.The blood was taken in all groups after intragastric administration for 6 weeks.Then the anti -double stranded DNA anti-body(dsDNA), antinuclear antibody (ANA), complement 3(C3), complement 4(C4), immunoglobulin M(IgM), immunoglobulin G(IgG) levels were measured by enzyme -linked immunosorbent assay (ELISA)method.In the spleen lymphocytes experiment , the dsDNA and ANA levels were measured in the lymphocyte supernatant after the effects of serum containing Langchuangqing granule on spleen lymphocytes.Results In the forssman cutaneous vasculitis reac-tion, compared with model group , the OD of the skin was significantly reduced in high dose test group in guinea pigs ( P<0.05 ).In the lupus experiment in MRL/lpr mice , compared with model group , high dose of langchuangqing granule significantly reduced dsDNA[(47.25 ±6.05) vs (37.51 ±3.26) ng· L-1] and IgM levels, improved C3 lev-els(P<0.05, P<0.01).Also in the middle dose group, IgM levels notably reduced and C3 increased(P<0.05,P<0.01 ).In the spleen lymphocytes experiment , the dsDNA levels in lymphocyte supernatant significantly reduced af-ter treated with the 20%drug-containing serum ( P<0.05 ).Conclusion The effects of Langchuangqing granule on the systemic lupus erythematosus may be related to the immunological functions of Autoantibodies , complement , immunoglobulin.关键词
狼疮清颗粒/系统性红斑狼疮/抗体/补体/免疫球蛋白Key words
Langchuangqing granule/systemic lupuserythematosus/antibody/complement/immunoglobulin分类
医药卫生引用本文复制引用
孙帅军,梁涛,陆叶,黄小玲,耿珊,马丽杰,许立..狼疮清颗粒对系统性红斑狼疮的动物实验研究[J].中国临床药理学杂志,2016,32(12):1125-1128,4.基金项目
江苏省科技厅科技支撑计划基金资助项目 （）
