中国临床药学杂志2016，Vol.25Issue(3)：153-156,4.
HPLC法测定大鼠血浆中米托蒽醌浓度及其在药动学中的应用
HPLC analysis of mitoxantrone concentration in rat plasma and its application in pharmacokinetic study
摘要
Abstract
AIM To develop a method for the determination of mitoxantrone in rat plasma by HPLC.METHODS The plasma sample preparation was precipitated with 10％ trichloroacetic acid-methanol solution.Chromatographic separation was carried out on a Xbridge-C18 column (150 mm× 2.1 mam,3.5 μm) with mobile phase consisted of 0.3 ％sodium heptanesulfonate solution (pH was adjusted to 2.0 with phosphoric acid) and acetonitrile (72:28).The flow rate was 1.0 mL· main-1,and the detection wavelength was 240 nm.Bupropion was used as internal standard.The established method was applied to pharmacokinetic study of mitoxantrone in rats.RESULTS Excellent linear calibration curve of mitoxantrone was obtained in the plasma concentration range of 100-10 000 μg· L-1 (r =0.999 6).The relative standard deviations of inter-and intra-day precisions were all less than 6％.The average recovery was above 80％.The relative standard deviations of short-term room temperature preservation and long-term cryopreservation stabilities were all less than 8％.Dosage of 5 mg· kg-1 mitoxantrone was given to rats by intravenous injection,its AUC(0→t) was (1 178.44±70.07) μg·h·L-1,t1/2z was (0.39±0.12) h,CL z/F was (3.91 ±0.26) L-1·h·kg-1,Vz was (2.18 ± 0.63) L· kg-1,tmax was (0.22 ± 0.04) h and ρmax was (2 380.91 ± 360.12) μg· L-1 respectively.CONCLUSION The method is simple,specific and reliable for the pharmacokinetic research of mitoxantrone in rats.关键词
HPLC/米托蒽醌/血浆浓度/药动学Key words
HPLC/mitoxantrone/plasma concentration/pharmacokinetics引用本文复制引用
周子晔,周彬,王陈翔,龚大湖,黄爱芳,张秀华..HPLC法测定大鼠血浆中米托蒽醌浓度及其在药动学中的应用[J].中国临床药学杂志,2016,25(3):153-156,4.基金项目
温州市科技计划项目(编号Y20140235) （编号Y20140235）
