中国实验动物学报2016，Vol.24Issue(3)：239-242,247,5.DOI:10.3969/j.issn.1005-4847.2016.03.004
裸小鼠肝癌原位移植模型的建立
Establishment of an orthotopic implantation model of hepatocellular carcinoma in nude mice
摘要
Abstract
Objective To establish a new orthotopic implantation model of hepatocellular carcinoma in BALB/c nude mice. Methods The nude mice were under general anesthesia with isoflurane inhalation. Hepatocellular carcinoma was induced by subcutaneous inoculation of human hepatocellular carcinoma Bel-7402 cells in nude mice. The orthotopic implantation model of hepatocellular carcinoma in nude mice was established by orthotopic implantation of small pieces of the liver cancer tissues into the mouse liver by either a sandwich method or embedding method. The post-operative tumor growth was determined by a small animal in vivo imaging system. Histopathological examination of the liver and tumor tis-sues was performed at four weeks after modelling. Results The orthotopic hepatocellular carcinoma model of nude mice established by the sandwich method had several distinct advantages: shorter operative time, less operational difficulties, with a high rate of tumor formation, no wide-spread peritoneal tumor dissemination, well-preserved tumor histological struc-ture, and low animal mortality. Conclusions Compared with the embedding method, the sandwich method can save much time for preparation of this orthotopic implantation model of hepatocellular carcinoma in nude mice, and provide a more convenient platform for research of liver cancer pharmacodynamics and other biological studies.关键词
BALB/c裸鼠/肝癌/原位移植/小动物活体成像系统Key words
BALB/c nude mice/Liver cancer/Orthotopic implantation/Small animals living imaging system分类
生物科学引用本文复制引用
田树红,王日超,邢桂兰,肖敏,符健..裸小鼠肝癌原位移植模型的建立[J].中国实验动物学报,2016,24(3):239-242,247,5.基金项目
国家自然科学基金资助项目（NO 81160408）。 （）
