中国药房2016，Vol.27Issue(19)：2618-2621,4.DOI:10.6039/j.issn.1001-0408.2016.19.09
野生与栽培黄管秦艽7种指标成分的比较研究
Comparative Study on 7 Indicative Constituents of Wild and Cultivated Gentiana officinalis
摘要
Abstract
OBJECTIVE:To compare the contents of loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopi-croside,sweroside,isoorientin and isovitexin in wild and cultivated Gentiana officinalis,and to provide basis for rational use of G. officinalis. METHODS:UPLC method was adopted. The separation was performed on ACQUITY UPLC® BEH C18 column (50 mm × 2.1 mm,1.7 μm) with mobile phase consisted of methanol-0.04% phosphoric acid (gradient elution) at the flow rate of 0.3 ml/min. The detection wavelength was set at 242 nm,and column temperature was 30 ℃. RESULTS:For loganic acid,swertiama-rin,6′-O-β- D-glucosyl gentiopicroside,gentiopicroside,sweroside,isoorientin and isovitexin,a good linearity was obtained in the range of 2.1-537.1 μg,1.05-270 μg,0.92-236 μg,11.1-2 830 μg,0.75-192 μg,0.167-102 μg,0.216-52.80 μg(r≥0.999 5), respectively. Their average recoveries were 97.72%-99.84%(RSD≤3.39%,n=6). The contents of loganic acid,swertiamarin, 6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside and isoorientin in the wild sample were higher than in the cultivat-ed;the content of isovitexin was lower than the cultivated,but there was no statistical significance(P>0.05). The sum of gentiopi-croside and loganin acid content were all higher than 2.5% in both wild and cultivated samples,and met the requirements of 2015 edition of Chinese Pharmacopoeia(first part). CONCLUSIONS:The content difference of 7 indicative constituents in wild and cul-tivated G. officinalis is not statistically significant,and the indicative constituents of the pharmacopoeia is qualified.关键词
野生/栽培/黄管秦艽/超高效液相色谱法/指标成分Key words
Wild/Cultivated/Gentiana officinalis/UPLC method/Indicative constituents分类
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杨燕梅,马晓辉,吕培霖,卢有媛,林丽,王振恒,晋玲,朱田田..野生与栽培黄管秦艽7种指标成分的比较研究[J].中国药房,2016,27(19):2618-2621,4.基金项目
中央本级重大增减支项目子课题（No.20603020212， No.20603020110）；2015年甘肃省高等学校科研项目 （）
