中国药房2016，Vol.27Issue(19)：2622-2625,4.DOI:10.6039/j.issn.1001-0408.2016.19.10
五味子抗氧化活性组分筛选
Screening of Antioxidant Active Components of Schisandra chinensis
摘要
Abstract
OBJECTIVE:To screen antioxidant active components of Schisandra chinensis. METHODS:The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity(IC50)as index and ethanol volume frac-tion,material-liquid ratio and extraction time as factors,and the verification test were made. The fractions(SC-0,SC-10,SC-30, SC-50,SC-70,SC-95) were made by extracting and purifying S. chinensis with macroporous resin with water and 10%,30%, 50%,70%and 95%ethanol. With IC50 and total antioxidant capacity(determined by ABTS method)as indexes(vitamin C as pos-itive control),the antioxidant active components of S. chinensis were optimized. The contents of 5 kinds of lignan in different posi-tions of S. chinensis were determined by HPLC. RESULTS:The optimal extraction condition of S. chinensis was as follows as 60% ethanol,material-liquid ratio of 1∶14,extracting for 2.0 h. The average IC50 of DPPH free radical scavenging activity was 23.81 mg/ml(RSD=0.52%,n=3)in verification test. SC-0 did not have antioxidant abilities. DPPH free radical scavenging activi-ty of those components (ie. the IC50 value from low to high) were in the following order of positive control>SC-50>SC-30>SC-95>SC-70>SC-10;total antioxidant ability of them were in the following order of SC-50>positive control>SC-30>SC-70>SC-95>SC-10;the contents of 5 types of lignan in different components were in the following order of SC-70>SC-50>SC-95>SC-30. CONCLUSIONS:The antioxidant active component of S. chinensis is 50%ethanol eluate.关键词
五味子/抗氧化/活性组分/提取工艺/正交试验/洗脱物/DPPH自由基清除能力/总抗氧化能力Key words
Schisandra chinensis/Antioxidation/Active components/Extraction technology/Orthogonal test/Eluate/DPPH free radical scavenging activity/Total antioxidant ability分类
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金银萍,侯微,高薇,刘俊霞,王玉帅,焉石,王英平..五味子抗氧化活性组分筛选[J].中国药房,2016,27(19):2622-2625,4.基金项目
吉林省科技发展计划项目 （）
