中国药房2016，Vol.27Issue(19)：2684-2686,3.DOI:10.6039/j.issn.1001-0408.2016.19.29
紫苏叶提取液的澄清纯化工艺优选
Optimization of Clarification and Purification Technology of Perillae folium Extract
王柏强 1刘福 2何效平 1曾芝兰 2罗飞 1罗巧林1
作者信息
- 1. 川北医学院附属医院药剂科，四川南充637000
- 2. 川北医学院药学院，四川南充 637000
- 折叠
摘要
Abstract
OBJECTIVE:To optimize the clarification and purification technology of Perillae folium extract. METHODS:The effects of 3 clarification and purification methods as chitosan flocculation clarification,ZTC 1+1-Ⅱflocculation clarification,water precipitation on retention rate of total flavonoids and removal rate of solid of Perillae folium extract were compared to screen suit-able clarification and purification technology. With the retention rate of total flavonoids and removal rate of solid as comprehensive evaluation index,single factor and orthogonal test were designed to investigate the optimal value of concentration proportion,the amount of the flocculant,flocculation temperature and whisking speed in optimal clarification and purification method. RESULTS:Among 3 methods,the chitosan flocculation clarification was the best with concentration proportion of 1∶4,chitosan of 1.0 g/L, flocculation temperature at 60 ℃,whisking speed of 100 r/min,whisking time of 4 min,standing time of 12 h. Under the condi-tion of optimal processing,the retention rate of total flavonoids was (85.1 ± 0.75)%,and the removal rate of solid was (24.6 ± 1.33)%(n=5). CONCLUSIONS:Chitosan flocculation can be used to effectively remove the impurity of Perillae folium extract, and optimized clarification and purification technology is stable and feasible.关键词
紫苏叶提取液/壳聚糖/ZTC 1+1-Ⅱ/澄清纯化工艺/总黄酮/正交试验Key words
Perillae folium extract/Chitosan/ZTC 1+1-Ⅱ/Clarification and purification technology/Total flavonoids/Or-thogonal test分类
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王柏强,刘福,何效平,曾芝兰,罗飞,罗巧林..紫苏叶提取液的澄清纯化工艺优选[J].中国药房,2016,27(19):2684-2686,3.
