中国药业2016，Vol.25Issue(12)：64-68,5.
降压类中成药及保健食品中非法添加氯沙坦的定性定量检测方法研究
Qualitative and Quantitative Method Study of Losartan Illegally Added into Antihypertensive Traditional Chinese Medicine and Health Food
摘要
Abstract
Objective To establish a qualitative and quantitative method for the detection of losartan illegally added into antihypertensive traditional Chinese medicines and health food. Methods Qualitative screening of losartan illegally added into antihypertensive traditional Chinese medicine and health food was firstly performed by UPLC-Q-TOF-MS/MS. The qualitative analysis was performed on a Shi-madzu Shim-pack XR-ODS column(100 mm × 2. 0 mm,2. 2 μm) with the mobile phase of acetonitrile and 0. 5% formic acid by gradient elution,the flow rate was 0. 3 mL/min. Electrospray ionization source(ESI) was operated in positive and negative mode. The scan range was from 100 to 1 000 m/ z. The quantitative detection of positive sample detected by UPLC-Q-TOF-MS/MS was used by HPLC,Chromatographic separation was achieved on a Waters XBridgeTM C18 column (150 mm × 4. 6 mm,5 μm) with the mobile phase of acetonitril and 0. 2% phosphate by gradient elution. The detection wavelengths was 220 nm and the flow rate was 1. 0 mL/min. Results Sampling from the market in 9 batches of Chinese patent medicines and health food diet,there were 2 batch samples detected illegally added losartan. The losartan content was 48. 3,20. 9 mg/g. Conclusion The method is rapid,accurate and highly sensitive, which can be used as a supplementary test method of antihypertensive traditional Chinese medicines and health food illegally added losartan.关键词
中成药/保健食品/非法添加/氯沙坦/超高效液相色谱-四级杆-飞行时间串联质谱联用Key words
Chinese patent medicine/health food/illegal added/losartan/UPLC-Q-TOF-MS/MS分类
医药卫生引用本文复制引用
马春艳,封淑华,冯丽,段琼,胡伟杰..降压类中成药及保健食品中非法添加氯沙坦的定性定量检测方法研究[J].中国药业,2016,25(12):64-68,5.基金项目
河北省食品药品监督管理局科技重点项目，项目编号ZD2014006。 （）
