中国药业2016，Vol.25Issue(13)：8-10,3.
姜黄素对心房颤动大鼠心房肌细胞Kir2．1，KvLQT1，HERG基因表达的影响
Effect of Curcumin on Kir2(1, KvLQT1 and HERG Gene Expression in Atrial Myocytes of Rats with Atrial Fibrillation
摘要
Abstract
Objective To learn the effect of curcumin on Kir2. 1, KvLQT1 and HERG gene expression in atrial myocytes of rats with atrial fibrillation, and to investigate the effect and mechanism of curcumin in the treatment of atrial fibrillation. Methods 24 atrial fib-rillation rats were randomly divided into two groups, 12 in curcumin group and 12 in control group. The control group and the curcumin group were intervened by normal saline and curcumin solution respectively. 1 week later, the single atrial myocytes of rats were obtained by enzymatic method. Real time PCR and Western blot techniques were used to detect the mRNA expression of Kir2. 1 channel which affected inward rectifier potassium current ( IK1 ) , KVLQT1 channel which affected slow activation of delayed rectifier potassium current ( IKs ) , and HERG channel which affected fast activation of delayed rectifier potassium current ( IKr ); and internal reference gene was GAPDH. Results The expression level of mRNA of Kir2. 1 channel in curcumin group was down regulated, and the difference was sta-tistically significant ( t=2. 853, P < 0. 05 ) in comparison with control group;The mRNA expression level of KvLQT1 and HERG chan-nels in curcumin group were up regulated, and the difference was statistically significant ( t=1. 862, 1. 758, P < 0. 05 ) in comparison with control group. Conclusion The effect of curcumin on atrial fibrillation was induced by Kir2. 1, KvLQT1 and HERG gene expres-sion in atrial myocytes of rats with atrial fibrillation.关键词
姜黄素/心房颤动/心房肌细胞/基因表达Key words
curcumin/atrial fibrillation/atrial myocytes/gene expression分类
医药卫生引用本文复制引用
廖小香..姜黄素对心房颤动大鼠心房肌细胞Kir2．1，KvLQT1，HERG基因表达的影响[J].中国药业,2016,25(13):8-10,3.基金项目
湖南省科学技术厅科技计划2014年一般项目，项目编号2014FJ3118。 （）
