中国药业2016，Vol.25Issue(13)：10-12,3.
坏死性凋亡特异性抑制剂抗环孢素A致坏死性凋亡的机制研究
Mechanism of Necrostatin-1 on Necroptosis Induced by Cyclosporin A
摘要
Abstract
Objective To explore the protective effect mechanism of necrostatin-1 ( Nec-1 ) , a specific inhibitor of necroptosis, on nephrotoxicity of cyclosporin A ( CsA ) in vivo. Methods 30 C57/BL6 male mice were randomly divided into the control group, CsA group and Nec-1 group. The control group was treated with olive oil. The CsA group was treated with CsA 100 mg/kg · d by gavage. The Nec-1 group was treated with Nec-1 2. 5 mg/kg 0. 5 h before treated with CsA. After 14 d of treatment, the content change of SCr, BUN were determined, the activity change of GSH and content change of MDA in the cortex were determined. The expressions of RIP3 in the cortex were detected. Results After 14 d of treatment, compared with the control group, the levels of SCr, BUN were increased, the activity of GSH in the cortex deceased, and MDA level increased in the CsA group;the levels of SCr, BUN were obviously decreased, the activity of GSH increased, and MDA level decreased in the Nec-1 group;however, the expression of RIP3 in the cortex of the 3 groups showed no significant difference. Conclusion Nec-1 has obvious protective effect on nephrotoxicity in-duced by CsA. Necroptosis underlies mechanism of the toxicity of CsA.关键词
环孢素A/坏死性凋亡特异性抑制剂/坏死性凋亡Key words
cyclosporin A/necrostatin-1/necroptosis分类
医药卫生引用本文复制引用
欧阳资章,王秋玲,刘晓萍,陈方,肖诚胤,赖香茂,钟志华,阮劲,邓惠容,江晟..坏死性凋亡特异性抑制剂抗环孢素A致坏死性凋亡的机制研究[J].中国药业,2016,25(13):10-12,3.基金项目
广东省医学科学技术研究基金，项目编号A201484016060888。 （）
