中国药业2016，Vol.25Issue(13)：22-24,3.
罗汉松实多糖含量测定方法研究
Content Determination of Podocarpus Macrophyllus
摘要
Abstract
Objective To determine the polysaccharides content of fruit from Podocarpus macrophyllus. Methods The ultrasonic-assisted extraction method was used to extract polysaccharides from the fruit of Podocarpus macrophyllus and the polysaccharides content was determined by sulphuric acid-phenol colorimetric method, with glucose as reference substance. The UV detection wavelength was 490 nm. Results Glucose absorbance showed a good linear relationship with the content in the range of 20-100 μg/mL. The regression equation is Y=0. 008 0 X-0. 0158, r=0. 998 0 ( n=5 ) , and the average recovery rate was 101. 64%, RSD=4. 77%( n=6 ) . The aver-age content of polysaccharides in Podocarpus macrophyllus is 4. 74 mg/g ( according to glucose ) . Conclusion This determination method is simple to be operated, with good reproducibility, and the results are accurate and reliable, which can be used to determine the polysaccharides content of Podocarpus macrophyllus.关键词
罗汉松/多糖/含量测定/超声辅助提取Key words
Podocarpus macrophyllus/polysaccharides/content determination/ultrasonic-assisted extraction分类
医药卫生引用本文复制引用
庞逸敏,林彩琴,潘娇红,蒋伟哲,黄增琼..罗汉松实多糖含量测定方法研究[J].中国药业,2016,25(13):22-24,3.基金项目
广西南校中青年教师基础能力提升项目，项目编号KY2016YB096；广西南宁市生物制药产业大型科学仪器与设备共享与服务平台建设-广西医科大学共享平台建设，项目编号20121052－1。 （）
