安徽大学学报（自然科学版）2016，Vol.40Issue(4)：87-91,5.DOI:10.3969/j.issn.1000-2162.2016.04.014
LY333531对高糖高脂下系膜细胞分泌细胞外基质的影响
Effect of LY333531 on the expression of extracellular matrix in mesangial cells exposed to high glucose and lysophosphatidylcholine
摘要
Abstract
To investigate the effect of PKCβinhibitor (LY333531) on extracellular matrix (ECM ) of masangial cells in high glucose ( HG ) and lysophosphatidylcholine (LPC ) environment ,human mesangial cells (HMCs) were cultured alone and divided into 4 groups :control ;LY333531 ;HG and LPC(HG + LPC);HG ,LPC and LY333531(HG + LPC +LY333531) .First ,we used the method of quantitative real‐time PCR(qRT‐PCR) to detect the expression of PKCβI;then ,the levels of collagen IV and fibronectin were detected by enzyme linked immunosorbent assay (ELISA) in the supernatant .These results suggested that the mRNA expression of PKCβI in the group of HG+LPC was higher than the control group (P< 0 .05) ,and the effect could be obviously inhibited by LY333531 (P< 0 .05) . Meanwhile ,compared with the control group ,the level of collagen IV and fibronectin in the supernatant was significantly increased in the group of HG +LPC (P <0 .05) ,and the effect could be also inhibited by LY333531 notably (P<0 .05) .Therefore ,we could conclude that the HG+LPC can aggrandize the secretion of ECM by increasing the expression of PKCβI in mesangial cells , which can increase the risk of renal fibrosis , however , LY333531 can weaken the effect obviously .关键词
高糖高脂/系膜细胞/PKCβI/细胞外基质Key words
high glucose and lysophosphatidylcholine/mesangial cells/protein kinase C βI (PKCβI)/extracellular matrix (ECM )分类
医药卫生引用本文复制引用
何笑云,欧春麟,肖艳华,周素娴..LY333531对高糖高脂下系膜细胞分泌细胞外基质的影响[J].安徽大学学报（自然科学版）,2016,40(4):87-91,5.基金项目
国家自然科学基金资助项目（81260134，81560148）；广西科技厅青年基金资助项目 （）
