安徽农业大学学报2016，Vol.43Issue(3)：359-365,7.DOI:10.13610/j.cnki.1672-352x.20160512.012
核盘菌磷酸泛酰巯基乙胺基转移酶编码基因的预测与生物信息学分析
Prediction and bioinformatics analysis of phosphopantetheinyl transferase gene in Sclerotinia sclerotiorum
摘要
Abstract
Phosphopantetheinyl transferase (PPTase) is an important class protein superfamily existing in eukaryotes.To decipher the function of PPTases in the biological metabolism and pathogenicity of Sclerotinia sclerotiorum,the characters of PPTases genes and the encoded protein were predicted and analyzed using bioinformatics methods,including general physical and chemical properties,hydrophobicity,transmembrane region,signal peptide,conserved domains,secondary and tertiary structure,localization sites in cells,and the phylogenetic tree.Results showed that the candidate coding genes Ss-Pptl,Ss-Ppt2 and Ss-Ppt3 of PPTase in S.sclerotiorum were selected in the genome.The proteins encoded by the three genes were hydrophilic and nonsecreting proteins.There were lots of alpha helix and randon coil in the secondary and tertiary structure of the three proteins.Additionally,all of the three proteins had either SCOP or ACPS domains,or both.So we speculated that Ss-Pptl,Ss-Ppt2 and Ss-Ppt3 might be the PPTases of S.sclerotiorum.This work could provide a cue for further study on the function of the PPTase in S.sclerotiorum.关键词
核盘菌/磷酸泛酰巯基乙胺基转移酶/生物信息学Key words
Sclerotinia sclerotiorum/phosphopantetheinyl transferase/bioinformatics分类
生物学引用本文复制引用
李秀丽,樊淼,吴亚,张茜茹,高智谋..核盘菌磷酸泛酰巯基乙胺基转移酶编码基因的预测与生物信息学分析[J].安徽农业大学学报,2016,43(3):359-365,7.基金项目
国家公益性行业(农业)科研专项(201103016),安徽省现代产业(油菜)体系专项和2014年宿州区域发展协同创新中心全国开放课题(2014SZXTKF04ZD)共同资助. （农业）
