安徽农业大学学报2016，Vol.43Issue(3)：366-372,7.DOI:10.13610/j.cnki.1672-352x.20160512.029
酵母乙醇提取物对重组大肠杆菌外源蛋白表达的影响
Effect of Yeast extract on heterologous protein production in recombinant Escherichia coil
摘要
Abstract
The ethanol extract of yeast contains a variety of active substances extracted from beer yeast.Preliminary study found that the ethanol extract can significantly promote protein expression in E.coli.Our laboratory used E.coliBL21 (DE3) / pGEX-4t-1-ggt as the main research bacteria and added 2 g·L-1 lactose to the medium as an inducer to study the role of yeast alcohol extract on protein expression of γ-GGT.We found that γ-GGT protein expression yield and the growth of the host bacteria can be significantly improved when the final concentration at 10 g·L-1 of yeast extract was batch added into medium containing 2 g·L-1 of lactose at different fermentation periods.The results showed that the amount of expressed protein increased by 10 ％～50％ comparing with that induced by 1 mmol·L-1 IPTG and cell density increased by 1.75-3 times.Similar effect has been shown for different proteins expressed in recombinant Escherichia coli.The mixture of ethanol yeast extract and lactose may be the effective alternative inducer to replace toxic IPTG.This result would lay a foundation for large-scale protein expression in the future.关键词
酵母乙醇提取物/乳糖/诱导/蛋白合成Key words
yeast extract/lactose/induce/protein synthesis分类
生物科学引用本文复制引用
朱岩岩,熊爱英,桑秀梅,王晶,王期,殷志敏..酵母乙醇提取物对重组大肠杆菌外源蛋白表达的影响[J].安徽农业大学学报,2016,43(3):366-372,7.基金项目
江苏省科技厅产学研联合创新资金—前瞻性研究项目(BY2013001-03)资助. （BY2013001-03）
