安徽农业大学学报2016，Vol.43Issue(3)：373-377,5.DOI:10.13610/j.cnki.1672-352x.20160512.023
利用曲霉sp.HS-6发酵玉米秸秆进行固体纤维素酶的制备
Production of solid cellulose through fermentation of maize straw by Aspergillus sp.HS-6
摘要
Abstract
In this paper,we explored the production conditions of cellulase through fermentation of maize straw by Aspergillus sp.HS-6 with the addition of NH4Cl,(NH4)2SO4,NH4NO3,KNO3,and CO(NH2)2 as nitrogen sources at three concentrations (0.5,1 and 2 g·L-1).The optimal ferment0tion time,the optimal nitrogen source and Aspergillus sp.HS-6 concentration were determined.Based on the optimum fermentation conditions,salting-out and freeze-drying techniques were used to produce solid enzymes.The results showed that:the optimal fermentation time was 3 days by determining the enzyme activity and the optimal nitrogen source was NH4NO3 at 0.5 g·L-1.Under this condition,we obtained the product of solid cellulase with a recovery rate of 48.0％.This experiment would provide some references for a large-scale production of cellulase in the future.关键词
玉米秸秆/曲霉/纤维素酶/氮源/发酵Key words
Aspergillus/cellulose/nitrogen source/maize straw/fermentation分类
化学化工引用本文复制引用
薛银婷,何兴兵,林永慧,黄诗,罗永路..利用曲霉sp.HS-6发酵玉米秸秆进行固体纤维素酶的制备[J].安徽农业大学学报,2016,43(3):373-377,5.基金项目
国家自然科学基金(31360135,31560205,41501335),湖南省高校重点实验室平台开放基金(12K107),2013年湖南省大学生研究性学习和创新性实验计划项目(237)和湖南省战略性新兴产业科技攻关项目(2014GK1062)共同资助. （31360135,31560205,41501335）
