安徽农业科学2016，Vol.44Issue(15)：1-4,9,5.
分子印迹预处理在红霉素检测中的应用研究
Research on Application of Molecular Imprinting Pre-treatment in Erythromycin Detection
摘要
Abstract
Objective]The aim was to establish a method for rapid detection of erythromycin in samples.[Method] Based on the precipitation method to prepare the molecularly imprinted polymer(MIP), the effects of different dispersed phases on preparation and adsorption properties of MIP were investigated.The adsorption properties of the polymer was further evaluated by Scatchard model.The erythromycin MIP adsorption specific in its structural analogs as well as common antibiotics in actual food samples were explored and the spiked recovery experiments with milk and egg samples were conducted.[Result] The results showed that when the ratio of template molecule, functional monomer and cross linking agent was 1∶4∶20, the adsorption properties of MIP was the best and the imprinting factor could reach 3.45.The imprinted polymer which obtained by this method reached the adsorption equilibrium only need 5 h, and the maximum adsorption capacity could reach 56 μg/g, greatly reducing the polymer balanced time.The average recovery ratio in milk and egg samples could reach 82.99%-98.09%.[Conclu-sion] This study provides a new method for rapid detection of erythromycin, simplifies the detection steps and improves the detection efficien-cy.This new method has broad application prospects.关键词
红霉素/分子印迹/富集特异性Key words
Erythromycin/Molecular imprinting/Enrichment specific分类
农业科技引用本文复制引用
胡琪,姚卫蓉,谢云飞..分子印迹预处理在红霉素检测中的应用研究[J].安徽农业科学,2016,44(15):1-4,9,5.基金项目
国家自然科学基金项目（21203076）；江苏省双创人才计划项目。 （）
