安徽农业科学2016，Vol.44Issue(15)：24-27,30,5.
灰绿青霉阿魏酸酯酶的分离纯化和酶学性质研究
Study on Separation,Purification and Enzymatic Properties of Ferulic Acid Esterase from Penicillium glaucum
摘要
Abstract
Objective] The aim was to study purification and enzymatic properties of feruloyl esterase in the culture solution of Penicillium glau-cum XGY36.[Method] The feruloyl esterase was obtained in the culture solution of Penicillium glaucum XGY36 and was purified to be homoge-neous by the steps of ammonium sulfate precipitation,DEAE-cellulose DE52 chromatographies,and Sephadex G-75 chromatography.The enzyme protein with high purity was obtained,enzymatic properties were studied.[Result] Purification of about 6.54 fold was achieved with an overall yield of 59.7%.The molecular weight of purified feruloyl esterase was estimated to be about 36.5 ku by SDS-PAGE.The optimum temperature and pH of the enzyme activity were 50 ℃and 5.0,respectively in catalytic reaction of ethyl ferulate.The enzyme activity was stable under 60 ℃, and with pH range of 3.0-7.0.The activity of feruloyl esterase was highly enhanced by Zn2 +,Mg2 +and Ca2 +,whereas it was strongly inhibited by the metal ions Pb2 +,Hg2 +and Ag +,while Na +,K +,Mn2 +,Cu2 +and Fe2 +had no strong effect on feruloyl esterase activity.[Conclusion] Fer-uloyl esterase has potential applied value and fit to apply in the fields of food,medicine and biology etc..关键词
灰绿青霉/阿魏酸酯酶/纯化/酶学性质Key words
Penicillium glaucum/Ferulic acid esterase/Purification/Enzymatic properties分类
生物科学引用本文复制引用
徐大伟,高兆建,张铁柱,杨杰,王东星,杜永凯..灰绿青霉阿魏酸酯酶的分离纯化和酶学性质研究[J].安徽农业科学,2016,44(15):24-27,30,5.基金项目
大学生创新创业基金项目（201311998053Y）；徐州市科技计划项目（XF13C011，XF13C027）。 （）
