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尾巨桉3个无性系外植体诱导技术研究

石前 梁秀莉 李飞宇 邓冬丽 李丽芳 李俏

安徽农业科学2016,Vol.44Issue(15):164-166,215,4.
安徽农业科学2016,Vol.44Issue(15):164-166,215,4.

尾巨桉3个无性系外植体诱导技术研究

Study on Explant Induction of Different Hybrids of Eucalyptus urophylla ×E.grandis Clones

石前 1梁秀莉 1李飞宇 2邓冬丽 1李丽芳 1李俏1

作者信息

  • 1. 广西壮族自治区国有东门林场,广西扶绥532108
  • 2. 中种国际种子有限公司,北京100028
  • 折叠

摘要

Abstract

Objective] The aim was to study explant induction relevant technology of Eucalyptus urophylla ×E.grandis three clones.[Meth-od] The optimal collection season, sampling part, disinfection time and concentration of plant growth regulator(6-BA) was selected, so as to im-prove germination rate of explants of Eucalyptus urophylla × E.grandis clones.[ Result] The spring and autumn were the optimal seasons for the explant induction, semi-lignified branches was the most suitable part of explant sampling , the most appropriate disinfection duration with mer-curic chloride is 7 minutes for DH32-26 and DH32-28, and 5 minutes for DH32-29.6-BA1.0 mg/L and NAA0.4 mg/L as plant growth regulator combination was good for explant induction which with the best germination rate.[Conclusion] In order to improve the successful rate of explant induction, a comprehensive consideration should be made in terms of the collection season, sampling part, disinfection time and plant growth reg-ulator combination.The study can provide theoretical basis for rapid propagation of Eucalyptus urophylla × E.grandis seedlings.

关键词

尾巨桉/无性系/外植体诱导

Key words

Eucalyptus urophylla ×E.grandis/Clone/Explant induction

分类

农业科技

引用本文复制引用

石前,梁秀莉,李飞宇,邓冬丽,李丽芳,李俏..尾巨桉3个无性系外植体诱导技术研究[J].安徽农业科学,2016,44(15):164-166,215,4.

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