安徽农业科学2016，Vol.44Issue(16)：114-116,3.
黔产苦参ISSR-PCR反应体系正交优化研究
Orthogonal Optimization of ISSR-PCR Reaction System of Suphora flavercens Ait
摘要
Abstract
Objective]To select a suitable method of ISSR-PCR reaction system for Suphora flavercens Ait. [Method] Modified CTAB method was used to extract the genomic DNA from fresh young leaves of S. flavercens. The suitable ISSR-PCR reaction system was established by orthogo-nal design L16(45) based on the Mg2+, dNTPs, primer,template DNA and Taq DNA. [Result]The optimal conditions in 25μL ISSR-PCR system were 90 ng DNA template, 0. 4μmol/L primer, 2. 0 mmol/L Mg2+, 0. 5 U TaqDNA Polymerase, and 0. 5 mmol/L dNTPs. [Conclusion]The es-tablished ISSR-PCR reaction system is verified by 19 samples of S. flavercens, which verifies that this system is stable and reliable, and can be used for the analysis of genetic diversity of S. flavercens.关键词
苦参/CTAB/ISSR-PCR反应体系/正交设计Key words
Suphora flavercens Ait/CTAB/ISSR-PCR reaction system/Orthogonal test分类
农业科技引用本文复制引用
金燕,龙庆德,常楚瑞,陆平祝,王晓丽..黔产苦参ISSR-PCR反应体系正交优化研究[J].安徽农业科学,2016,44(16):114-116,3.基金项目
贵州省中药现代化科技产业研究开发专项(黔科合ZY字[2012]3004号) （黔科合ZY字[2012]3004号）
贵州省科技计划项目(黔科合重大专项字[2015]6009-2)。 （黔科合重大专项字[2015]6009-2）
