安徽农业科学2016，Vol.44Issue(16)：157-159,3.
芦笋“冠军”花药培养愈伤组织的诱导
The Callus Induction of Asparagus“Champion” Anther Culture
包艳存 1李书华 1李霞 1李芳1
作者信息
- 1. 山东省潍坊市农业科学院生物所，山东潍坊261041
- 折叠
摘要
Abstract
Objective] The aim was to study asparagus“champion” anther culture technique. [Method] Using the F1 hybrid asparagus variety“champion” as the test material, callus induction in tissue culture process and effects of growth regulator on callus differentiation were studied. [Result] The results showed that using 0. 1% mercuric chloride sterilization 5 min, inoculated on the MS solid medium by adding NAA0. 8 mg/L + 6-BA 2. 0 mg/L of darkness first 10 days, and then light culture, anther callus induction rate reached about 16. 5%. [Conclusion] The asparagus“champion” anther culture technical system is established, which will lay a foundation for carrying out asparagus all male breeding.关键词
芦笋“冠军”/花药培养/愈伤诱导Key words
Asparagus officinalis“Champion”/Anther culture/Callus induction分类
农业科技引用本文复制引用
包艳存,李书华,李霞,李芳..芦笋“冠军”花药培养愈伤组织的诱导[J].安徽农业科学,2016,44(16):157-159,3.
