安徽农业科学2016，Vol.44Issue(16)：179-182,4.
尾巨桉3个无性系继代增殖培养研究
Multiplication Subculture of Three Clones of Eucalyptus urophylla × E. grandis
石前 1李飞宇 2梁秀莉 1邓冬丽 1李丽芳 1兰俊1
作者信息
- 1. 广西壮族自治区国有东门林场，广西扶绥532108
- 2. 中种国际种子有限公司，北京100028
- 折叠
摘要
Abstract
Objective] To research the multiplication subculture technology of three clones of Eucalyptus urophylla × E. grandis, and to pro-vide theoretical foundation for the rapid propagation of E. urophylla × E. grandis seedlings. [ Method] With three clones (DH32-26, DH32-29, DH32-28) as the test materials, the optimal combination of regulators was screened, as well as the coagulator, dark culture days and culture flask. Thus, multiplication coefficient was relatively high. [Result] The optimal combination of plant growth regulators for subculture of three clones were DH32-26(6-BA 0. 5 mg/L + NAA 0. 2 mg/L), DH32-28(6-BA 0. 4 mg/L + NAA 0. 2 mg/L) and DH32-29(6-BA 0. 3 mg/L +NAA 0. 2 mg/L). DH32-26 multiplication coefficient showed no significant differences in culture medium with carrageenan and agar as the coag-ulators. Subculture multiplication coefficients of DH32-28 and DH32-29 in culture medium with carrageenan as the coagulator was higher than those with agar as the coagulator. During the process of rooting, culture medium with carrageenan as the coagulator had far better rooting than that with agar as the coagulator. In the initial stage of DH32-26 and DH32-28 subculture, black cloth was used for shading for 5-10 d; while DH32-29 did not need all dark culture. DH32-29 subculture in wild-mouth bottle reduced the production cost of seedlings; but DH32-26 and DH32-28 were not suitable for the subculture in wild-mouth bottle. [Conclusion] To enhance the multiplication coefficient of clones, we should select the optimal regulators, coagulator, dark culture days and culture flask.关键词
尾巨桉/无性系/继代增殖Key words
Eucalyptus urophylla × E. grandis/Clones/Multiplication分类
林学引用本文复制引用
石前,李飞宇,梁秀莉,邓冬丽,李丽芳,兰俊..尾巨桉3个无性系继代增殖培养研究[J].安徽农业科学,2016,44(16):179-182,4.
