安徽农业科学2016，Vol.44Issue(17)：7-8,90,3.
不同龄期果蝇DNA含量的检测与比较
Detection and Comparison of DNA Content in Fruit Flies in Different Periods
摘要
Abstract
Objective] The aim was to determine DNA content in 8 fruit flies at different ages.[Method] Total DNA was extracted from Ⅰ,Ⅱ, Ⅲ instar larva, pupa, imago of 7 mutant lines of Drosophila virilis and Drosophila melanogaster.DNA content was determined by ultravio-let visible spectrophotometer, PCR detection was conducted on DNA.[Result] The contents of DNA in eight fruit flies I, II and III instar lar-va were increased, and the contents of DNA in most of the pupa and imago were decreased;the efficiency of PCR of fruit flies in five periods were all good, imago was the best, larva was better.[ Conclusion] The DNA quality of larva, pupa and imago all can ensure the experiments carry out such as the PCR amplification, molecular cloning, species identification and so on.关键词
果蝇/龄期/DNA含量/PCRKey words
Fruit flies/Age/DNA content/PCR分类
农业科技引用本文复制引用
黄莉娜,王剑峰,高成,刘广纯..不同龄期果蝇DNA含量的检测与比较[J].安徽农业科学,2016,44(17):7-8,90,3.基金项目
国家自然科学基金项目（31201743，31372245）；辽宁省教育厅科学研究项目（12013452）。 （）
