安徽农业科学2016，Vol.44Issue(17)：34-38,43,6.
槲皮素－AAPH氧化产物结构鉴定及其对 HepG2细胞氧化还原状态的影响
Structure Identification of the Quercetin Oxidation Products Induced by AAPH and Effects on the Redox State of HepG2 Cells
温斐婷 1成向荣 1陈侃俊 1王菲 1乐国伟 1施用晖1
作者信息
摘要
Abstract
Objective] The aim was to explore how the quercetin oxidation products induced by AAPH influence the redox state of HepG2 cells.[ Method] Quercetin was mixed with APPH as oxidant under 37 ℃ for 12 h.The structural characterization were analyzed by UV scan and LC-MS.HepG2 cells were treated with reaction product of quercetin-AAPH and incubated for 24 h.Viable cells were harvested, and the cell viability, ROS level, glutathione ( GSH) and glutathione disulphide ( GSSG) content, malandialdehyde ( MDA) level, total antioxidant capacity ( T-AOC) , superoxide dismutase ( SOD) and catalase ( CAT) activity were assayed with the appropriate test kits, the oxidant stress related genes were assayed by DNA microarray.[Result] The chromatogram showed peaks eluting at 294 nm after 12 h reaction and the struc-tural analysis result indicated that mixture of four oxidation products were obtained in the reaction system.Compared with the blank group, Quercetin-AAPH oxidation products elevated the ROS and MDA generation, and reduced cell viability, T-AOC, CAT, GSH-Px and SOD and GSH/GSSG activity significantly.Nrf2, NQO-1, Prdx expression level was down-regulated and Keap-1, Nox-2, Cox-2 expression level was up-regulated in the meanwhile.[Conclusion] These findings indicate that quercetin oxidation products could prompt HepG2 cells to generate more oxidative stress.关键词
槲皮素/氧化产/HepG2细胞/氧化应激Key words
Quercetin/Oxidation products/HepG2 cells/Oxidative stress分类
生物科学引用本文复制引用
温斐婷,成向荣,陈侃俊,王菲,乐国伟,施用晖..槲皮素－AAPH氧化产物结构鉴定及其对 HepG2细胞氧化还原状态的影响[J].安徽农业科学,2016,44(17):34-38,43,6.
