安徽农业科学2016，Vol.44Issue(17)：96-100,5.
酶解法提取白鲢鱼油的工艺研究及品质分析
Technology Research and Quality Analysis of Fish Oil from Silver Carp by Enzyme Hydrolysis Method
摘要
Abstract
Objective] To provide method and reference for the extraction of freshwater fish oil and the comprehensive utilization of processing by-products of freshwater fish.[Method] Fish oil was extracted from the belly part of silver carp by enzyme hydrolysis method.We researched the effects of enzyme type,liquid-solid ratio,hydrolysis temperature,pH and enzyme dosage on the extraction rate of fish oil .Then,the physicochemi-cal property and fatty acid composition of obtained crude fish oil were analyzed .[ Result] Alkaline protease had the optimal extraction effects on fish oil from belly part.Under the condition of 1.0∶1 liquid-solid ratio,the response surface optimization was carried out .The optimal technology was as follows:54 ℃ hydrolysis temperature,8 200 U/g enzyme dosage,pH 8.6 and 3 h enzymolysis time.Under this condition,the extraction rate of fish oil was 64.32%.The fish oil peroxide value was 5.48 mmol/kg; iodine value was 1 204.2 g/kg; saponification value was 169.21 mg/g and acid value was 3.56 mg/g.Among them,contents of EPA and DHA were 6.54% and 4.53%,respectively.The n-3/n-6 was 2.89. [Conclusion] The fish oil indexes reach the first grade standard of crude fish oil,which has high nutritional value and broad application prospect.关键词
鱼油/酶解/提取/脂肪酸组成Key words
Fish oil/Enzymolysis/Extraction/Fatty acid composition分类
农业科技引用本文复制引用
骆婷,许学勤,夏文水,姜启兴,许艳顺..酶解法提取白鲢鱼油的工艺研究及品质分析[J].安徽农业科学,2016,44(17):96-100,5.基金项目
国家大宗淡水鱼产业技术体系建设专项项目（CARS－46）。 （）
