安徽农业科学2016，Vol.44Issue(17)：146-150,5.
假蒟不同部位5种次生代谢产物含量测定
Determination of Five Kinds of Secondary Metabolites in Different Parts of Piper sarmentosum Roxb
摘要
Abstract
Objective] To establish the analytical methods for detecting the contents of total flavonoids, total alkaloids , total saponins, total phenolics and total tannins in different parts of Piper sarmentosum Roxb.[ Method] The content of total flavonoids was measured by the NaNO2A-l( NO 3) 3-NaOH colorimetry.The content of total alkaloids was measured by the bromocresol green acid dyes colorimetric assay .The content of total spaonins was measured by the vanillin-acetic acid dyes colorimetric assay.The content of total phenolics was measured by the Folin-Ciocalteu assay.The content of total tannins was measured by the phospho molybdenum tungstic acid -casein colorimetry.[ Result] The average recovery of rutin was 98.11% and RSD was 0.59%, the contents of total flavonoids in roots, stems and leaves of P.sarmentosum were 0.25%, 0.33% and 1.18% respectively.The average recovery of berberine hydrochloride was 99.62% and RSD was1.52%, the con-tents of total alkaloids in roots, stems and leaves of P.sarmentosum were 0.01%, 0.03% and 0.19% respectively.The average recovery of ginsenoside Re was 99.21% and RSD was 1.06 %, the contents of total saponins in roots, stems and leaves of P.sarmentosum were 4.13%, 1.71% and 4.33% respectively.The average recovery of gallic acid was 97.95% and RSD was 0.88 %, the contents of total phenol in roots, stems and leaves of P.sarment soum were 0.68%, 1.08% and 2.42% respectively.The average recovery of gallic acid was 97.92%and RSD was 3.14%, the contents of total tannins in leaves, stems and roots of P.sarmentosum were 0.72%, 1.21% and 1.69% respec-tively.[Conclusion] These five proposed methods are precise, accurate and reproducible, and they are suitable for the determination of total flavonoids, total alkaloids, total saponins, total phenolics and total tannins in P.sarmentosum.关键词
假蒟/不同部位/次生代谢产物/含量测定Key words
Piper sarmentosum Roxb./Different parts/Secondary metabolites/Content determination分类
农业科技引用本文复制引用
张燕,张洪斌..假蒟不同部位5种次生代谢产物含量测定[J].安徽农业科学,2016,44(17):146-150,5.基金项目
2013年海南热带海洋学院植物学课程群教学团队。 （）
