北京中医药大学学报2016，Vol.39Issue(7)：568-574,7.DOI:10.3969/j.issn.1006-2157.2016.07.009
麻黄、五味子联用地塞米松对肺纤维化大鼠血管新生促进因子与抑制因子的影响
Effects of Herba Ephedra and Fructus Schisandrae combined with dexam-ethasone on angiogenic factors and inhibitors in rats with idiopathic pul-monary fibrosis
摘要
Abstract
Objective To observe the effects of Mahuang ( Herba Ephedra ) and Wuweizi ( Fructus Schisandrae) combined with dexamethasone ( DXM) on expression of angiogenic factors and inhibitors in rats with idiopathic pulmonary fibrosis ( IPF) .Methods Wistar male rats of SPF level ( n=106 ) were divided randomly into sham operation group , model group, Mahuang group, Wuweizi group, Mahuang+DXM group , Wuweizi+DXM group and DXM group .The model of IPF was established by using instilla-tion of bleomycin (7 mg/kg) except for the sham operation group .Each group was administered intra-gastrically of corresponding drug .All rats were sacrificed on the 7th and 28th day via abdominal aorta blood-letting, and the pathological changes of lung tissue were evaluated with HE staining and the expres -sion of hypoxia-inducible factors 1α( HIF-1α) , platelet derived growth factor ( PDGF) , pigment epitheli-um-derived factor ( PEDF) and endostatin in rat serum were also measured with ELISA .Results Com-pared with the sham operation group , the expression of HIF-1α,PDGF, PEDF and endostatin in model group significantly increased (P<0.01);the HIF-1αcontent on the 28th day was higher than that on the 7th day, and the PEDF and endostatin content were lower than that on the 7th day.The expression of HIF-1αand PDGF significantly decreased in Mahuang group and Wuweizi group compared with the model group, and the expression of PEDF and endostatin increased (P<0.05).Compared with Mahuang group on the 28th day,the expression of HIF-1αand PDGF on the 7th day increased markedly, but the expres-sion of PEDF and endostatin on the 28th day was lower than those on the 7th day.In the Wuweizi group the changes in the expression of HIF-1α,PDGF, PEDF and endostatin were opposite to Mahuang group.The expression of HIF-1αand PDGF significantly decreased in Mahuang+DXM group and Wuweizi+DXM group compared with the model group ( P<0 .05 ) , and the expression of PEDF and endostatin increased . In Wuweizi+DXM group the HIF-1αcontent was significantly higher than those in DXM group while the PEDF content was significantly lower ( P<0.05 ) .Conclusion Regulatory factors on angiogenesis lose their balance in rats with IPF .Mahuang and Wuweizi could achieve inhibition of the expression of HIF-1αand PDGF, and selectively promote the expression of PEDF and endostatin .When combined with DXM, the selectivity might be weakened in different pathological stages .关键词
麻黄/五味子/地塞米松/特发性肺纤维化/血管新生/大鼠Key words
Mahuang(Herba Ephedra)/Wuweizi(Fructus Schisandrae)/dexamethasone/idiopathic pul-monary fibrosis/angiogenesis/rats分类
医药卫生引用本文复制引用
张小娟,张田,田伟兰,李睿,翟华强..麻黄、五味子联用地塞米松对肺纤维化大鼠血管新生促进因子与抑制因子的影响[J].北京中医药大学学报,2016,39(7):568-574,7.基金项目
国家自然科学基金资助项目 （）
