北京中医药大学学报2016，Vol.39Issue(7)：575-579,5.DOI:10.3969/j.issn.1006-2157.2016.07.010
补肾益肺消癥方对特发性肺纤维化大鼠Caspase-12信号通路关键分子基因和蛋白表达的影响
Effects of Bushen Yifei Xiaozheng Decoction on the expression of key mo-lecular genes and proteins in Caspase-12 signaling pathway in IPF rats
摘要
Abstract
Objective To investigate the effects of Bushen Yifei Xiaozheng Decoction ( kidney-tonifying lung-boosting stasis-resolving decoction ) on the expression of key genes and proteins in Casepase-12 signaling pathways in rats with idiopathic pulmonary fibrosis ( IPF ) .Methods IPF was induced with bleomycin in rats except for the normal group .Pirfenidone was used as positive control , while Bushen Yifei Xiaozheng Decoction ( TCM ) was administered during ( TCM preventive group ) and after ( TCM treatment group ) the model established and throughout the whole experiment ( TCM preventive and treat-ment group ) .Pathological change in the rat lungs was observed with Masson staining , meanwhile the ex-pression of molecular genes and proteins involved in Caspase-12 signaling pathways was detected by using Western blot and Real-time PCR.Results Masson staining revealed that the degree of fibrosis in TCM groups improved significantly and alveolar structure changed little . Protein and gene expression of Caspase-12 pathway in the model group was higher than those of normal group (P<0.05).In addition, the protein and gene expression of pirfenidone group and TCM groups were less than the model group ( P<0.05 or P<0.01), especially TCM preventive and treatment group .Conclusion Bushen Yifei Xi-aozheng Decoction could inhibit the process of pulmonary fibrosis via acting on the gene expression of Caspase-12 pathway and releasing the endoplasmic reticulum stress .关键词
补肾益肺消癥方/特发性肺纤维化/内质网应激/凋亡通路/含半胱氨酸的天冬氨酸蛋白水解酶-12/大鼠Key words
Bushen Yifei Xiaozheng Decoction ( kidney-tonifying lung-boosting stasis-resolving decoction )/idiopathic pulmonary fibrosis/endoplasmic reticulum stress/apoptosis pathways/Caspase-12/rats分类
中医学引用本文复制引用
杨颖溪,柴立民,吴甜甜,高伟华,朱紫亨,徐昉,余佳骏,晏军..补肾益肺消癥方对特发性肺纤维化大鼠Caspase-12信号通路关键分子基因和蛋白表达的影响[J].北京中医药大学学报,2016,39(7):575-579,5.基金项目
国家自然科学基金资助项目 （）
