吉林大学学报（医学版）2016，Vol.42Issue(4)：648-652,5.DOI:10.13481/j.1671-587x.20160403
PI3K-Akt 通路对老年心肌缺血预适应模型大鼠线粒体通透性转换孔开放程度的影响
Effect of PI3K-Akt signaling pathway on openness degree of mitochondrial permeability transition pore in aged model rats of myocardial ischemic preconditioning
摘要
Abstract
Objective: To study the effect of PI3K-Akt signaling pathway on the openness degree of mitochondrial permeability transition pore (mPTP)in the aged model rats of myocardial ischemic preconditioning (IPC),and to clarify its possible mechanism.Methods:Thirty-five Wistar Wistar rats (aged 21-23 months)were randomly divided into ischemia reperfusion (I/R)group,I/R+Wort (Wortmannin,inhibitor of PI3K)group,IPC group and IPC+ Wort group (n=7).The models of I/R and IPC were established and 0.6 mg·kg-1 Wort were injected to the caudal veins of the rats in Wort group before reperfusion. After 120 min reperfusion, the myocardium tissue protein was extracted, and the Akt and p-Akt protein expression levels were detected by Western blotting method.The myocardial mitochondrial of rats was separated through differential centrifugation, and the superoxide level, SOD level and openness degrees of mPTP were determined by microplate reader. Results:Compared with I/R group (0.288±0.071),the expression level of p-Akt protein in myocardium tissue of the rats in IPC group (0.346±0.051)was increased (P <0.05),and the expression level of p-Akt im myocardium tissue of the rats in I/R+ Wort group (0.044 ± 0.010)was decreased (P < 0.01).Compared with I/R group (0.216± 0.024),the superoxide level in mitochondrial of the rats in IPC group (0.187 ± 0.022)was decreased (P <0.05),and the superoxide level in mitochondrial in I/R + Wort group (0.218 ± 0.029)had no significant change (P >0.05).Compared with I/R group (1.15±0.15),the SOD activity in mitochondrial of the rats in IPC group (1.39±0.14)was increased (P <0.05),and the SOD activity in mitochondrial of the rats in I/R+ Wort group (1.17±0.21)had no significant change (P >0.05).Compared with I/R group,the mPTP openness degree in IPC group was decreased (P <0.05)during 6 to 2 min,however the openness degree in I/R+Wort group had no significant change (P > 0.05).Compared with IPC group,the mPTP openness degree in IPC+ Wort group was increased (P < 0.05).Conclusion:The IPC of aged rats can inhibit the openness of mitochondrial permeability transition pore through activating the PI3K-Akt signaling pathway.关键词
缺血再灌注/缺血预适应/磷脂酰肌醇 3 激酶/蛋白激酶 B/线粒体通透性转换孔Key words
ischemia reperfusion/ischemic preconditioning/phosphatidylinositol-3 kinase/protein kinase B/mitochondrial permeability transition pore分类
医药卫生引用本文复制引用
韦艳红,刘晓冬,刘艳,陆丽娟..PI3K-Akt 通路对老年心肌缺血预适应模型大鼠线粒体通透性转换孔开放程度的影响[J].吉林大学学报（医学版）,2016,42(4):648-652,5.基金项目
国家自然科学基金资助课题 （）
