吉林大学学报（医学版）2016，Vol.42Issue(4)：653-658,6.DOI:10.13481/j.1671-587x.20160404
卵泡抑素对肿瘤恶病质小鼠骨骼肌消耗的影响及其作用机制
Effect of follistatin on skeletal muscle wasting of cancer cachexia mice and its mechanism
摘要
Abstract
Objective: To observe the effects of follistatin (FST)on the skeletal muscle wasting of cancer cachexia mice and the expressions of Mstn, LncRNA-MALAT1 and Caspase-3, and to elucidate its associated molecular mechanisms.Methods:Thirty-two BALB/c mices were randomly assigned into:healthy control (HC) group,FST prevention (FP)group,FST treatment (FT)group and cancer cachexia (CC)group.The murine colon adenocarcinoma CT26 cells were inoculated subcutaneously into the mices in FP, FT and CC groups to establish the cancer cachexia models. The body weight, spontaneous activity and tumor growth were daily monitored.The mice in FP and FT groups were administrated with FST intraperitoneally on day 6 and 12 after inoculation.The samples were collected on day 20.The tumor and gastrocnemius weights of the mice were detected. The biochemical metabolism indexes and myofiber cross-sectional area of gastrocnemius tissue were detected.The mRNA expression levels of Mstn,Caspase-3 and LncRNA-MALAT1 were examined by Real-time PCR.The protein expression levels of Mstn and Caspase-3 were measured by Western blotting method. Results:Compared with CC group,the body weights,spontaneous activities,gastrocnemius weights and myofiber cross-sectional areas were increased (P <0.05);the serum levels of glucose,total protein and albumin of the mice in FP and FT groups were increased (P <0.05).The protein and mRNA expression levels of Mstn and Caspase-3 in gastrocnemius of the mice in CC group were significantly higher and the expression level of LncRNA-MALAT1 was significantly lower than those in HC group (P < 0.05).The mRNA and protein expression levels of Mstn and Caspase-3 in FP and FT groups were reduced and the expression level of LncRNA-MALAT1 was increased compared with CC group (P < 0.05).The prevention effect in FP group is better than FT group (P < 0.05). Conclusion:FST may alleviate the muscle wasting of the mice with cancer cachexia by inhibiting the expression of Mstn,thus upregulating the expression of LncRNA-MALAT1 which in turn to suppress the expression of Caspase-3.关键词
肿瘤恶病质/卵泡抑素/肌肉生长抑制素/长链非编码 RNA/肺癌转移相关转录本 1Key words
cancer cachexia/follistatin/myostatin/long non-coding RNA/metastasis-associated lung adenocarcinoma transcript1分类
医药卫生引用本文复制引用
王朝义,王强,郑曰勇,李聪,郭敦伟,唐华..卵泡抑素对肿瘤恶病质小鼠骨骼肌消耗的影响及其作用机制[J].吉林大学学报（医学版）,2016,42(4):653-658,6.基金项目
国家自然科学基金资助课题 （）
