吉林大学学报（医学版）2016，Vol.42Issue(4)：665-670,6.DOI:10.13481/j.1671-587x.20160406
异氟醚对转 APP 基因小鼠脑内海马神经元蛋白质损伤和蛋白聚集的影响
Influence of isoflurane in neuronal protein damage and aggregation in APP transgenic mouse hippocampus
摘要
Abstract
Objective:To observe the influence of inhaled anesthetic isoflurane in the neuronal protein damage and aggregation in the APP transgenic mouse hippocampus,and to investigate the intervention effect of trehalose. Methods:Sixty APP transgenic mice aged 12 months were divided into control group,isoflurane group (Iso group) and trehalose group (Tre group)(n=20).The rats in control group were not given any drugs and were put into the anesthetic box with continuonsly entering 2 L·min-1 oxygen for 2 h;the rats in Iso group and Tre group were respectively injected intraperitoneally with 2 mL saline or trehalose (400 μg·kg-1 )30 min before anesthesia,and then inhalated 1.4% isoflurane for 2 h.6 h after anesthesia,the hippocampus tissue of the mice was prepared,and DCFH-DA fluorescence was applied to detect the reactive oxygen species (ROS)level; 24 h after anesthesia, immunohistochemical method and Western blotting method were used to detect the contents of carbonyl compounds and nitrotyrosine and the Aβ1-42 protein expression level in hippocampus;TEM was applied to observe the formation of protein aggregates; TUNEL staining was performed to observe the apoptotic rate of hippocampal neurons. Results:Compared with control group, the ROS level, the expression levels of oxidative protein carbonyl compounds and nitrotyrosine,the expression level of Aβ1-42 protein,and the apoptotic rate of hippocampal neurons in Iso group were significantly increased (P < 0.05);compared with Iso group,the ROS level,the expression levels of oxidative protein carbonyl compounds and nitrotyrosine,the Aβ1-42 protein expression level, and the apoptotic rate of hippocampal neurons in Tre group were significantly decreased (P < 0.05).Conclusion:Isoflurane can induce the protein damage and aggregation,the apoptotic rate of hippocampal neurons,aggravate oxidative stress reaction,increase the apoptotic rate of brain hippocampal neurons in the APP transgenic mice;trehalose can intervene the neurotoxicity induced by inhaled anesthetics.关键词
异氟醚/阿尔茨海默病/蛋白质损伤/细胞凋亡/海藻糖Key words
isoflurane/Alzheimer’s disease/protein damage/apoptosis/trehalose分类
医药卫生引用本文复制引用
刘楠,冯春生,朴美花,刘明,孙宇田..异氟醚对转 APP 基因小鼠脑内海马神经元蛋白质损伤和蛋白聚集的影响[J].吉林大学学报（医学版）,2016,42(4):665-670,6.基金项目
国家自然科学基金资助课题（81271215，81141065）；吉林省科技厅科技发展计划项目资助课题 （）
