吉林大学学报（医学版）2016，Vol.42Issue(4)：671-675,5.DOI:10.13481/j.1671-587x.20160407
当归挥发油对自发性高血压大鼠心肌组织中微小 RNA差异表达谱的影响
Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats
摘要
Abstract
Objective:To explore the effect of Angelica Sinensis Volatile Oil on the mi RNA expression profiling in myocardium tissue of the spontaneously hypertensive rats (SHRs),and to clarify the possible mechanism of Angelica Sinensis Volatile Oil.Methods:All the SHRs were divided into Angelica group,model group and captopril group,and other Wistar rats with the same age were selected as control group.The non-invasive systolic blood pressure was detected;after 4 weeks of administration,the changes of miRNA expression profiling in myocardium tissue were measured by Affymetrix miRNA 4.0 Array.KEGG analysis was used to identify the target genes. Results:Compared with control group,the systolic blood pressure of the rats in model group was significantly decreased (P < 0.05)before treatment.4 weeks after administration,compared with model group,the systolic blood pressure of the rats in Angelica group was significantly decreased (P <0.05).Compared with model group, 29 differential miRNAs of rats in Angelica group were found (P < 0.05 ), with 13 up-regulated miRNAs and 16 down-regulated miRNAs.The KEGG analysis results showed that miR-19a,let-7i,and miR-181c were related to insulin signaling pathways; let-7i, miR-181a, and miR-455 were related to VEGF signaling pathways;miR-122,miR-181a, miR-200b, miR-181c, let-7i, and miR-19a were related to apoptosis (P < 0.05 ). Conclusion:Angelica Sinensis Volatile Oil can decrease the blood pressure in SHRs and it can regulate the blood pressure by regulating the miRNA related to insulin signaling pathways and VEGF signaling pathways.关键词
当归挥发油/大鼠,近交 SHR/微小 RNA 表达谱Key words
Angelica Sinensis Volatile Oil/rat,inbred SHR/miRNA expression profiling分类
医药卫生引用本文复制引用
伊琳,陈蓓蓓,孙少伯,杨陇,纪禄风,石向慧..当归挥发油对自发性高血压大鼠心肌组织中微小 RNA差异表达谱的影响[J].吉林大学学报（医学版）,2016,42(4):671-675,5.基金项目
国家自然科学基金资助课题（81460669）；甘肃省科技厅第十批科技计划项目资助课题 （）
