吉林大学学报（医学版）2016，Vol.42Issue(4)：681-684,4.DOI:10.13481/j.1671-587x.20160409
MT01对牙龈卟啉单胞菌感染成骨细胞 MG63中Ⅰ型胶原mRNA 表达的促进作用及其意义
Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance
摘要
Abstract
Objective:To investigate the effect of MT01 on the differentiation of osteoblasts under infected condition through determining the expression level of collagen Ⅰ (Col Ⅰ )mRNA in MG63 cells treated with Porphyromonas gingivalis (Pg).Methods:The cultured MG63 cells were divided into blank control,MT01,Pg, and MT01+Pg groups.MT01 at a concentration of 1 mg·L-1 was added into the MG63 cells,and the cells were incubated for 3 h.The cells treated with PBS (1 mg·L-1 )were used as control group.Then Pg (MOI=100∶1) was added.Real-time PCR was used to detect the expression levels of ColⅠ mRNA in MG63 cells at 2,4,6,8, 12 and 24 h after incubation.Results:Compared with blank control group,the levles of ColⅠ mRNA in the MG63 cells in MT01 and MT01 + Pg groups had no significant changes at 2 and 4 h (P > 0.05);the Col Ⅰ mRNA expression levels in MT01 group at 8,12 and 24 h were increased (P < 0.05 or P < 0.01).Compared with Pg group,the expression levels of ColⅠ mRNA in MT01+ Pg at 2 and 4 h were decreased,but the expression levels of ColⅠ mRNA were increased at 6,8,12 and 24 h (P <0.05 or P <0.01).Conclusion:MT01 can up-regulate the expression level of Col Ⅰ mRNA in the infected MG63 cells; MT01 could promot the differentiation of osteoblasts under infected condition.关键词
单链寡脱氧核苷酸 MT01/牙龈卟啉单胞菌/人成骨细胞/Ⅰ型胶原Key words
specific sequence oligodeoxynucleotide MT01/Porphyromonas gingivalis/human osteoblast cell/collagen Ⅰ分类
生物科学引用本文复制引用
刘引,申玉芹,高涵,费鸿博,胡天琦,李洋洋,顾中一,林崇韬..MT01对牙龈卟啉单胞菌感染成骨细胞 MG63中Ⅰ型胶原mRNA 表达的促进作用及其意义[J].吉林大学学报（医学版）,2016,42(4):681-684,4.基金项目
国家自然科学基金面上项目资助课题（81371153）；吉林省科技厅自然科学基金面上项目资助课题 （）
