吉林大学学报（医学版）2016，Vol.42Issue(4)：685-689,5.DOI:10.13481/j.1671-587x.20160410
MNU 法诱导大鼠原位膀胱癌模型的建立及 MRI 的诊断价值
Establishment of rat model of orthotopic bladder cancer induced by MNU and diagnosis value of MRI
摘要
Abstract
Objective:To construct the rat models of orthotopic bladder cancer induced by N-methyl-nitrosourea (MNU),and to evaluate the value of magnetic resonance imaging (MRI)in the noninvasive diagnosis of the bladder cancer model.Methods:Sixty femail SD rats were divided into experiment group (n=45)and control group (n=15).The rats experiment group were induced with MNU (2 mg per rat)by intravesical administration every other week,for 4 times.Meantime,the rats in control group were treated with normal saline (0.2 mL per rat)by intravesical administration.At the end of the 14th week,all rats were examined by MRI and the pathological changes of bladder tissue were detected.Results:In experiment group,43 rats were alived and 2 rats were died at the end of the 14th week;the survial rate was 95.6% and the death rate was 4.4%;the abnormal signals were found in each of 43 rats by MRI which manifested as bladder tumor, and the same results were identified by pathology;the tumor formation rate was 100%.In control group,14 rats were alived and 1 rat was died at the end of the 14th week;the survival rate was 93.3%,and the death rate was 6.7%;there was no abnormal signal in the MRI examination and no bladder cancer in the pathological examination;the tumor formation rate was 0.The tumor formation rates of bladder cancer of the rats in two groups had significant difference (P <0.05),and the death rate had no significant difference (P >0.05).Conclusion:The method to establish the rat models of orthotopic bladder cancer induced by MNU is simple and reliable;the results of MRI are consistent with the pathological results and MRI examination is a reliable diagnostic method concerning this model.关键词
疾病模型,动物/膀胱肿瘤/N-甲基亚硝基脲/磁共振成像/活体鉴定Key words
disease model,animals/bladder neoplasms/N-methyl-nitrosourea/magnetic resonance imaging/identification of living body分类
医药卫生引用本文复制引用
宋志强,沈海山,沈俊,吴建臣,张明,李然伟,张继珍,李胜文..MNU 法诱导大鼠原位膀胱癌模型的建立及 MRI 的诊断价值[J].吉林大学学报（医学版）,2016,42(4):685-689,5.基金项目
国家自然科学基金青年科学基金资助课题（81201527）；清华-周大福医学研究专项基金资助课题（202836019-04）；清华-裕元医学科学研究基金资助课题 （）
