吉林大学学报（医学版）2016，Vol.42Issue(4)：742-745,4.DOI:10.13481/j.1671-587x.20160421
高活性小牛血去蛋白提取物对小鼠酒精性肝损伤的保护作用
Protective effect of high activity deproteinized extract of calf blood on alcohol liver injury of mice
摘要
Abstract
Objective:To observe the protective effect of deproteinized extract of calf blood (DECB)on the ethanol-induced liver injury of the mice,and to preliminaryly discuss its mechanism. Methods:Sixty healthy ICR mice were divided into control group,model group,positive drug group,low,medium and high doses of DECB groups (n=10).By intragastric administration,the mice in control group were given 20 mL·kg-1 saline solution, the mice in low,medium and high doses of DECB groups were administrated with 0.125,0.250,0.500 g·kg -1 DECB,and the mice in positive drug group were administrated with 0.63 g·kg -1 Hugan Tablets;once a day for 30 d. 1 h after the last administration,except control group,the mice in other groups were administrated with one-time grant of 50% ethanol 14 mL·kg -1 ,and fasted for 16 h to establish the models of acute alcohol liver injury.The endurance alcohol time and drunk time of the mice were determined,the activities of aspartate aminotransferase (ALT)and alanine transaminase (AST)activity in serum of the mice were detected,the levels of triglyceride (TG),glutathione (GSH)and malonic dialdehyde (MDA)in liver tissue were determined,and the pathological changes of liver tissue were detected.Results:Compared with model group,the drunk symptoms of the mice in different doses of DECB groups were obviously reduced,the endurance time of the mice in high dose of DECB group and positive drug group was prolonged (P <0.05),and the drinking time was shortened (P <0.05);the ALT and AST activities in serum in mediun and high doses of DECB groups were significantly lower than those in model group (P <0.05).Compared with model group,the MDA and TG levels in liver tissue of the mice in medium and high doses of DECB groups and positive drug group were obviously reduced,and the GSH levels were increased (P <0.05);compared with model group,the pathological damages of liver tissue of the mice in high dose of DECB group caused by ethanol were significantly reduced.Conclusion:DECB can improve ethanol-induced liver injury which may be related to the inhibition of hepatic oxidative stress response.关键词
高活性小牛血去蛋白提取物/酒精性肝损伤/氧化应激Key words
high activity deproteinized extract of calf blood/alcoholic liver injury/oxidative stress分类
医药卫生引用本文复制引用
石立强,韩笑,赵南晰,盛瑜,杜培革,安丽萍,陈丽娜,李洪宇,谢立亚,糜心雅,苑广信,孙晶波,王曼力,徐广宇..高活性小牛血去蛋白提取物对小鼠酒精性肝损伤的保护作用[J].吉林大学学报（医学版）,2016,42(4):742-745,4.基金项目
吉林省科技厅科技创新与科技成果转化计划基金资助课题（0150312014ZX）；吉林省卫计委科研项目资助课题（2014078）；吉林省发改委科研基金资助课题（2014Y103）；吉林省科技厅医药产业发展基金资助课题 （）
