滨州医学院学报2016，Vol.39Issue(3)：161-164,4.
基质交联分子-1调控前列腺癌细胞 PC-3中钙离子通道作用研究
The research of STIM1on store-operated calcium pathway in PC-3 prostate cancer cells
摘要
Abstract
Objective To investigate STIM1 real-time expression level and its role in adjusting calcium pathway when calcium concen-tration change in PC-3 prostate cancer cells .Methods Using adenovirus with STIM1-YFP fluorescent probe (105 viral particles per cell) we infected PC-3 prostate cancer cells and after 24 hours fluorescence microscope and cell count methods were used to detect the toxicol-ogy of virus probe on cell morphology and number .Real-time changes of STIM1-YFP were observed by using total internal reflection fluorescence microscope under three different conditions:addition of 100 micromoles sarcoplasmic reticulum calcium AT P enzyme inhibi-tor cyclopiazonic acid;calcium removal in extracellular buffer together with 2 micromoles EGTA ;addition of 100 micromoles carbachol . Results There was no significant difference in cell morphology and number after transfecting with STIM1 fluorescent probe .Fluores-cence intensity increased to (1.45 ± 0.2)(F/F0 ,n=18)after adding of 100 micromoles CPA .Fluorescence intensity increased to (1.38 ± 0.2)(F/F0 ,n=7)after adding of 2 millimoles EGTA .Fluorescence intensity increased to (1.62 ± 0.3)(F/F0 ,n=23)after adding of 100 micromoles carbachol .There are significant differences(P<0.01) .Conclusion STIM1 is expressed and active in PC-3 prostate cancer cells .STIM1 in polymer is formed on the membrane when the calcium concentration change in the cells inside and outside .The function of prostate cancer cells are adjusting by store-operated calcium pathway .关键词
基质交联分子-1/前列腺癌/钙池离子通道/钙离子Key words
STIM1/prostate cancer/store-operated calcium pathway/calcium分类
医药卫生引用本文复制引用
刘爽,乔令艳,张海利,刘楚,刘芳,米佳,田梗..基质交联分子-1调控前列腺癌细胞 PC-3中钙离子通道作用研究[J].滨州医学院学报,2016,39(3):161-164,4.基金项目
国家自然科学基金青年基金（81400771）；山东省自然科学基金（ZR2014HL028）；山东省高校科研发展计划（J14LE01）；滨州医学院科技重点计划 （）
