滨州医学院学报2016，Vol.39Issue(3)：165-168,4.
抑制 HOTAIR 的表达促进替莫唑胺引起的垂体腺瘤细胞凋亡
Silencing of HOTAIR expression inhibits the temozolomide-induced apoptosis in pituitary tumor cells
摘要
Abstract
Objective To investigate the effect of long non-coding RNA (lncRNA) HOTAIR on the temozolomide-induced apoptosis of pituitary tumor cells .Methods Pituitary adenoma cell line HP75 cells and GH3 cells were cultured in vitro ,quanti-tative real-time PCR was used to detect the expression level of lncRNA HOTAIR in HP75 cells and GH3 cells after treatment with temozolomide .The HP75 cells and GH3 cells were transfected with siRNA specially targeting HOTAIR to knock endoge-nous HOTAIR down in vitro ;furthermore ,MTT assay was used to detect the proliferation rate in HP75 and GH3 cells ,flow cytometry assay was employed to detect the temozolomide-induced apoptosis in HP75 cells and GH3 cells .Results Temozolo-mide could cause significant apoptosis in pituitary tumor HP75 cells GH3 cells ,at the same time ,the expression of HOTAIR was significantly reduced in cells treated with temozolomide;the expression of HOTAIR could be significantly inhibited via transfecting with siRNA targeting HOTAIR in HP75 cells and GH3 cells ;the knockdown endogenous HOTAIR could inhibit the proliferation rate in HP75 cells and GH3 cells ,and enhance the apoptosis rate in HP75 cells and GH3 cells treated with te-mozolomide .Conclusion Interference on the expression of HOTAIR in pituitary tumor cells could enhance the temozolomide-induced apoptosis of pituitary adenoma cells .关键词
HOTAIR/垂体腺瘤/替莫唑胺/凋亡Key words
HOTAIR/pituitary adenoma/temozolomide/apoptosis分类
医药卫生引用本文复制引用
李九州,张玉奇,赵全成,刘洪恩,左凯..抑制 HOTAIR 的表达促进替莫唑胺引起的垂体腺瘤细胞凋亡[J].滨州医学院学报,2016,39(3):165-168,4.基金项目
山东省医药卫生科技发展计划项目 （）
