重庆医学2016，Vol.45Issue(19)：2599-2601,3.DOI:10.3969/j.issn.1671-8348.2016.19.003
microRN A-34a在大鼠膝骨关节炎模型中的功能研究
Study on function of microRNA-34a in rat model of knee osteoarthritis
摘要
Abstract
Objective To explore the expression of microRNA‐34a(miR‐34a) in sodium iodoacetate‐induced rat model of os‐teoarthritis rat model and to initially clarify its function .Methods Thirty male Wistar rats were selected .The right knees were in‐jected with sodium iodoacetate (model group) and the left knees served as the control group without injecting by sodium iodoace‐tate .The expression levels of miR‐34a in the synovial fluid and articular chondrocytes were compared between two groups after con‐structing the model .Articular chondrocytes in the two groups were separated for conducting the primary culture ,after miR‐34a knockdown ,the flow cytometry was used to determine cell apoptosis .Results The Makin score in the model group was(5 .09 ± 1 .35)points ,which was (1 .27 ± 0 .64)points in the control group ,the difference had statistical significance (P<0 .05) .Compared with the control group ,the expression levels of miR‐34a in the synovial fluid and articular chondrocytes in the model group were in‐creased ,the difference was statistically significant(P<0 .05) .After the miR‐34a knockdown by using its complementary antisense fragment ,articular chondrocytes apoptosis in the model group was significantly inhibited (P<0 .05) .Conclusion The expression of miR‐34a in the synovial fluid of sodium iodoacetate‐induced knee osteoarthritis is increased ,moreover inhibiting the microRNA‐34a expression could reduce the apoptotic rate of articular chondrocytes in osteoarthritis rat .关键词
膝骨关节炎/关节液/微小RNA-34a/细胞凋亡Key words
knee osteoarthritis/synovial fluid/microRNA-34a/apoptosis分类
医药卫生引用本文复制引用
张文强,丁倩,张娜,李月白..microRN A-34a在大鼠膝骨关节炎模型中的功能研究[J].重庆医学,2016,45(19):2599-2601,3.基金项目
国家自然科学基金资助项目（81071520）。 （）
