重庆医学2016，Vol.45Issue(19)：2617-2619,3.DOI:10.3969/j.issn.1671-8348.2016.19.008
尿路感染分离金黄色葡萄球菌生物膜形成能力及相关基因的分析
Analysis of biofilm formation ability and related genes of Staphylococcus aureus isolated from urinary tract infections
摘要
Abstract
Objective To study the biofilm formation ability and related gene distribution of Staphylococcus (S .) aureus iso‐lated from urinary tract infections to provide the theoretical basis for the prevention and treatment of clinical infection .Methods The minimal inhibitory concentration was detected using the agar double dilution method .The bacterial adhesion ability was deter‐mined by flat colony counting method .The biofilm formation ability was analyzed by the 96‐well crystal violet staining method .The biofilm‐associated genes were detected by PCR amplification .Results Eleven clinical strains of S .aureus were high resistant to pen‐icillin and erythromycin ,whereas were all sensitive to vancomycin and nitrofurantoin .All the isolates had a strong ability of adhe‐sion ,but the biofilm formation ability was weak .Among them ,the icaAD and icaBC genes were amplified in 10 S .aureus isolates . Conclusion The adhesion ability and biofilm formation ability of S .aureus isolated from urinary tract infections have the strain differences ,and ica is an important gene of S .aureus biofilm formation .关键词
金黄色葡萄球菌/尿路感染/生物膜/黏附/ica基因Key words
Staphylococcus aureus/urinary tract infections/biofilm/adhesion/ica gene分类
医药卫生引用本文复制引用
孙凤军,洪海,冯伟,孙艺璇,夏培元..尿路感染分离金黄色葡萄球菌生物膜形成能力及相关基因的分析[J].重庆医学,2016,45(19):2617-2619,3.基金项目
国家自然科学基金资助项目（81373451）。 （）
