重庆医学2016，Vol.45Issue(21)：2881-2884,4.DOI:10.3969/j.issn.1671-8348.2016.21.001
不同程度弥漫性轴索损伤动物模型建立研究
Study on the establishment of rat model of different degree of diffuse axonal injury
摘要
Abstract
Objective To establish the different degrees of rat diffuse axonal injury (DAI) model by using a self‐made DAI device .Methods A total of 70 healthy adult clean SD rats were selected and randomly divided into the normal control group (n=10) and DAI group (n=60) ,then the DAI group was randomly subdivided into the group A ,B ,and C ,20 cases in each group .The rat head injury model was prepared by using the self‐made experimental device ,which made the rat head to simultaneously produce instant oversized linear and angular accelerations ,different degrees of rat DAI model ,including mild DAI(group A) ,moderate DAI (group B) and severe DAI(group C) ,were induced by different rotation back and forth ,accelerated movement times under the con‐stant air pressure .The pathological and behavior effect evaluation was performed .Results With the injury degree aggravating ,the time interval of nerve physiological reflex recovery and awakening time in the acute DAI groups were increased (P<0 .05) .The nerve function score after 7 d in the DAI groups was decreased (P<0 .05);the death rates within 14 d after injury in the group A , B and C were 5 .0% ,25 .0% and 50 .0% respectively .With the injury degree aggravating ,the DAI pathological characteristics were more significant .Conclusion This device could effectively establish different injury degrees of DAI animal model .关键词
弥漫性轴索损伤/实验装置/动物模型/大鼠Key words
diffuse axonal injury/experimental facility/animal model/rat分类
医药卫生引用本文复制引用
向玲,张雨婷,梁平,韦红,彭玲珑,李禄生..不同程度弥漫性轴索损伤动物模型建立研究[J].重庆医学,2016,45(21):2881-2884,4.基金项目
国家临床重点专科资助项目（X3257）；重庆市科委科技攻关应用技术研发类项目（cstc2012gg-yyjs10013）。 （）
