重庆医学2016，Vol.45Issue(21)：2894-2896,2899,4.DOI:10.3969/j.issn.1671-8348.2016.21.004
RIP1在急性胰腺炎胰腺腺泡细胞凋亡中的作用探讨
Study on role of RIP1 in apoptosis of pancreatic acinar cell in acute pancreatitis
摘要
Abstract
Objective To investigate the role of apoptosis and the regulating role of receptor interacting protein 1(RIP1) in acute pancreatitis .Methods Thirty C57 mice were divided into three groups :control group ,acute edematous pancreatitis (AEP) group and acute necrotizing pancreatitis (ANP) group .The AEP group was continuously injected by cerulein 50 μg/kg for 13 times ,the ANP group was continuously injected by cerulein 50μg/kg for 13 times and lipopolysaccharide 15 mg/kg once;the con‐trol group was injected by the same volume of normal saline for 7 times .The acinar cell apoptosis was observed by the terminal de‐oxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick‐end labeling (TUNEL) assay .The RIP1 mRNA expression was measured by real time fluorescence PCR .The expression of RIP1 protein was detected by Western blotting .Results The mouse models of AEP and ANP were established successfully .Compared with the control group ,acinar cell apoptosis existed in both AEP and ANP model groups ,moreover compared with the AEP group ,apoptosis in the ANP group were decreased ,the differences were statistically significant(P<0 .05) .Compared with the control group ,the expression of RIP1 mRNA and protein in the AEP group was increased ,while which in the ANP group were decreased ,the differences were statistically significant(P<0 .05) .Conclusion RIP1 participate in the pathogenesis of acute pancreatitis ,which may associate with acinar cell apoptosis .关键词
急性胰腺炎/水肿性胰腺炎/坏死性胰腺炎/细胞凋亡/受体相互作用蛋白Key words
acute pancreatitis/edematous pancreatitis/necrotizing pancreatitis/apoptosis/receptor interacting protein分类
临床医学引用本文复制引用
周翔宇,郑英强,何雪梅..RIP1在急性胰腺炎胰腺腺泡细胞凋亡中的作用探讨[J].重庆医学,2016,45(21):2894-2896,2899,4.基金项目
国家自然科学基金资助项目（81100272）；四川省科技厅基金资助项目（2014JY0004）。 （）
