重庆医学2016，Vol.45Issue(21)：2900-2902,3.DOI:10.3969/j.issn.1671-8348.2016.21.006
醛固酮诱导人肾小管上皮细胞胶原Ⅰ、Ⅲ表达的研究
Study on expressions of collagen Ⅰ and Ⅱ in aldosterone-induced human kidney tubular epithelial cells
摘要
Abstract
Objective To investigate the role of Rho kinase on collagen(COL)Ⅰ and Ⅲ expression of human renal tubular epithelial cells .Methods Human kidney tubular epithelial (HK‐2) cells were cultured in the RPMI‐1640 culture solution containing 15% fetal bovine serum .After 30 min pretreatment by the ALD receptor antagonist eplerenone(10 μmol/L) and Rho kinase inhibi‐tor Y27632(1 μmol/L) ,100 nmol/L ALD acted the HK‐2 cells for 24 h .The expressions of collagenⅠ and Ⅲ mRNA in each group were detected by real time PCR and the expression levels of COL Ⅰ ,Ⅲ and Rho kinase protein were detected by ELISA .Results ALD could up‐regulate the expressions of COLⅠ ,Ⅲ mRNA in HK‐2 cells ,and increased the levels of Rho kinase ,COLⅠ and Ⅲprotein ,while the Rho kinase inhibitor Y27632 and ALD receptor inhibitor eplerenone could antagonize these effects .Conclusion ALD could activate Rho kinase signal transduction pathway in HK‐2 cells and accelerate the progression of tubular interstitial fibro‐sis via Rho kinase induced expression of COL Ⅰ and Ⅲ .关键词
醛固酮/胶原Ⅰ/胶原Ⅲ/Rho激酶/人近端肾小管上皮细胞/肾小管间质纤维化Key words
aldosterone/collagen-Ⅰ/collagen-Ⅲ/Rho kinase/human proximal tubular epithelial cells/tubular interstitial fi-brosis分类
临床医学引用本文复制引用
黄海燕,魏佳莉..醛固酮诱导人肾小管上皮细胞胶原Ⅰ、Ⅲ表达的研究[J].重庆医学,2016,45(21):2900-2902,3.基金项目
海南省社会发展科技专项项目（2012SF04、2013SF04和2015SF43）。 （）
