重庆医学2016，Vol.45Issue(21)：2906-2908,2913,4.DOI:10.3969/j.issn.1671-8348.2016.21.008
miR-21对人喉鳞癌细胞Hep2迁移侵袭能力的影响
Effect of miR-21 on migration and invasion ability in human laryngeal squamous carcinoma cell Hep 2
刘永军 1管艳飞 1常顺2
作者信息
- 1. 云南省第一人民医院耳鼻喉科，昆明650032
- 2. 云南省第一人民医院神经外科，昆明650032
- 折叠
摘要
Abstract
Objective To explore the effect of microRNA‐21(miR‐21) on the migration and invasion ability in human laryn‐geal squamous carcinoma cell Hep2 .Methods The MTT method was used to detect the viability of Hep2 cells at 48 h after miR‐21 inhibitor and miR‐21 NC transferring into Hep2 cells by LipofectamineTM 2000 .The cell migration ability was detected by using the scratch test .The cell invasion ability was detected by using the Transwell method .The activation of phosphatase and tensin homo‐logue deleted on chromosome 10 (PTEN)/phosphatidylinositol 3 kinase (PI3K) /protein kinase B(Akt) signal pathway and the expression of matrix metalloproteinase 2 (MMP2) ,MMP9 ,reversion inducing cysteine rich protein with kazal motif (RECK) was detected by using the Western blotting .Results Compared with miR‐21 NC ,miR‐21 inhibitor could significantly reduce the Hep2 cellviability[(0.688±0.043)vs.(0.375±0.012)],inhibitedthemigrationability[(6.57±0.02)μm vs.(20.49±2.18)μm]and invasion ability[(100 .7 ± 10 .2) vs .(46 .8 ± 4 .3)] ,and the differences were statistically significant (P<0 .01) ,meanwhile miR‐21 inhibitor could down‐regulate the expression of PI3K ,MMP2 and MMP9(P<0 .01) ,and reduced the phosphorylation level of Akt (P<0 .01) ,up‐regulated the expression of PTEN and RECK (P<0 .01) .Conclusion miR‐21 inhibitor can significantly suppress the migration and invasion ability of Hep2 ,which may be related with the PTEN/PI3K/Akt signal pathway .关键词
微小RNA-21/人喉鳞癌 Hep2细胞/迁移/侵袭Key words
microRNA-21/human laryngeal squamous carcinoma cell Hep2/migration/invasion分类
临床医学引用本文复制引用
刘永军,管艳飞,常顺..miR-21对人喉鳞癌细胞Hep2迁移侵袭能力的影响[J].重庆医学,2016,45(21):2906-2908,2913,4.
