动物营养学报2016，Vol.28Issue(7)：2079-2089,11.DOI:10.3969/j.issn.1006-267x.2016.07.012
鹅脂肪甘油三酯脂肪酶和长链脂酰辅酶 A合成酶1基因表达差异及其对脂肪沉积和血清脂类代谢的调控
Differential Expression of Adipose Triglyceride Lipase and Long-Chain Acyl-CoA-Synthetase 1 Genes and Their Regulation of Fat Deposition and Serum Lipid Metabolism of Geese
摘要
Abstract
This experiment was conducted to study the difference of adipose triglyceride lipase ( ATGL) gene and long⁃chain acyl⁃CoA⁃synthetase 1 ( ACSL1) genes expression in different tissues and organs of geese, and to explore the expression of two genes on body fat deposition and serum lipid metabolism regulation. Selected thirty 16⁃week⁃old Wulong Geese ( half male and half female) , and the ATGL and ACSL1 gene expressions in different tissues and organs ( liver, heart, subcutaneous fat, abdominal fat, breast muscle, leg muscle, muscu⁃lar stomach, glandular stomach, small intestine, kidney, brain, lung, spleen ) were detected by real⁃time quantitative PCR after slaughter. The results showed as follows:1) The expressions of ATGL and ACSL1 genes were detected in subcutaneous fat, abdominal fat, liver, spleen, kidney, heart, breast muscle and leg muscle of geese; ATGL gene expression in subcutaneous fat and abdominal fat relative was the highest, followed by that in the liver and spleen, in kidney, heart, breast muscle and leg muscle only a few of expression. ACSL1 gene expression in subcutaneous fat, abdominal fat, liver and spleen was the highest, and slightly expressed in kidney, heart, breast muscle and leg muscle, and almost no expression in muscular stomach, glandular stom⁃ach and lung. 2) ATGL gene expression showed a significant negative correlation with intramuscular fat ( IMF) percentage in leg muscle, IMF percentage in breast muscle, percentage of abdominal fat, percentage of leg muscle and percentage of breast muscle (P<0.05 or P<0.01), and was significant positively correlated with percentage of subcutaneous fat ( P<0.05); ACSL1 gene expression was positively related with IMF percentage in leg muscle, IMF percentage in breast muscle and percentage of breast muscle ( P>0.05) , and was signifi⁃cantly positively correlated with percentage of leg muscle (P<0.05), while it showed a significant negative correlation with percentage of subcutaneous fat (P<0.05). 3) The ATGL gene expression was significantly positively correlated with the contents of total cholesterol ( TCH) , high density lipoprotein cholesterol ( HDL⁃C), low density lipoprotein cholesterol (LDL⁃C) and glucose (GLU) in serum (P<0.05 or P<0.01); the ACSL1 gene expression was negatively correlated with the content of TCH, HDL⁃C, LDL⁃C and GLU in ser⁃um( P>0.05) , and it were significantly negatively correlated with the triglyceride content. In conclusion, the expressions of ATGL and ACSL1 genes in different tissues and organs of geese have obvious differences, which have a negative regulation on fat deposition and serum lipid metabolism.关键词
鹅/脂肪甘油三酯脂肪酶基因/长链脂酰辅酶A合成酶基因/组织器官/基因表达/基因调控Key words
geese/ATGL gene/ACSL1 gene/tissues and organs/gene expression/gene regulation分类
农业科技引用本文复制引用
王宝维,孔敏,葛文华,张名爱,马传兴,张肖..鹅脂肪甘油三酯脂肪酶和长链脂酰辅酶 A合成酶1基因表达差异及其对脂肪沉积和血清脂类代谢的调控[J].动物营养学报,2016,28(7):2079-2089,11.基金项目
国家水禽产业技术体系专项基金（CARS-43-11）；山东省良种工程 （）
